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Whole cell iTRAQ proteome analysis of strain JA2

ABSTRACT: Here, we employed L-tryptophan metabolomics and iTRAQ based proteomic profiling of strain JA2 to decipher the molecular response. Wherein, L-tryptophan catabolism and molecular response of strain JA2 unexpected under chemotrophic conditions. Molecular response of L-tryptophan is essential to our understanding of L-tryptophan catabolism and can be demonstrated the physiological condition of microorganisms, when organisms was exposed with aromatic compounds ever changing the environment conditions. Cellular response such as membrane protein, RND and ABC pumps implicate to efflux of aromatic compounds metabolism of tryptophan by strain JA2. Furthermore, strain JA2 feed with tryptophan 210 differentially regulated proteins related signaling, transcription couple translation, stress, membrane transport and aromatic compounds metabolism highly upregulated. L-tryptophan metabolomics and global proteomic approaches rewiring the amino acid, fatty acid, lipid and energy of cells fed with tryptophan. Aromatic amino acid metabolism proteins upregulated and sulpher containing amino acid were down regulated leading toward the adaptation of L-tryptophan feed by strain JA2.

INSTRUMENT(S): Dionex instrument model

ORGANISM(S): Rubrivivax Benzoatilyticus Ja2 = Atcc Baa-35

TISSUE(S): Photosynthetic Cell

SUBMITTER: Md Shabbir Ahmad

LAB HEAD: Prof. CH VENNKATA RAMANA LAB

PROVIDER: PXD055514 | Pride | 2024-11-27

REPOSITORIES: Pride

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			Action	DRS
	1-JA-Strain.xlsx.xlsx	Xlsx
	iTRAQ6_01.raw	Raw
	iTRAQ6_02.raw	Raw
	iTRAQ6_03.raw	Raw
	iTRAQ6_04.raw	Raw

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