Dataset Information

Wolbachia modulation of lipid metabolism in Aedes albopictus mosquito cells

ABSTRACT: Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography FT-ICR mass spectrometry based lipidomicse DIMS and LCMS analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia compared to uninfected cells. Substantial shifts in the cellular lipid profile were apparent in the presence of Wolbachia. Most significantly, sphingolipids were depleted across all classes, and some reduction in diacylglyerol fatty acids and phosphatidylcholines was also observed. These lipid classes have previously been shown to be selectively enriched in DENV-infected mosquito cells, suggesting that Wolbachia may produce a cellular lipid environment that is antagonistic to viral replication. The data improve understanding of the intracellular interactions between Wolbachia and mosquitoes.

INSTRUMENT(S): LTQ FT Ultra

SUBMITTER: Ulf Sommer

PROVIDER: MTBLS210 | MetaboLights | 2016-03-11

REPOSITORIES: MetaboLights

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Wolbachia Modulates Lipid Metabolism in Aedes albopictus Mosquito Cells.

Molloy Jennifer C JC Sommer Ulf U Viant Mark R MR Sinkins Steven P SP

Applied and environmental microbiology 20160502 10

<h4>Unlabelled</h4>Certain strains of the intracellular endosymbiont Wolbachia can strongly inhibit or block the transmission of viruses such as dengue virus (DENV) by Aedes mosquitoes, and the mechanisms responsible are still not well understood. Direct infusion and liquid chromatography-Fourier transform ion cyclotron resonance (FT-ICR) mass spectrometry-based lipidomics analyses were conducted using Aedes albopictus Aa23 cells that were infected with the wMel and wMelPop strains of Wolbachia ...[more]

PMID: 26994075

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			Action	DRS
	MTBLS210	Other
	FILES	Other
	a_MTBLS210_DIMS	Other
	a_MTBLS210_LCMS	Other
	a_mtbls001_metabolite_profiling_mass_spectrometry.txt	Txt