Metabolomics

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Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality


ABSTRACT:

Horticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess quality characteristics in various pepper (Capsicum annuum L.) cultivars. DART-MS exhibited the ability to discriminate between pod colors and pungency based on chemical fingerprints, while REIMS could distinguish pepper market class (e.g., bell, lunchbox, and popper). Furthermore, DART-MS analysis resulted in the putative detection of important bioactive compounds in human diet such as vitamin C, p-coumaric acid, and capsaicin. The results of this study demonstrate the potential for these approaches as accessible and reliable tools for high throughput screening of pepper quality.

INSTRUMENT(S): Direct infusion MS - positive, Direct infusion MS - negative

SUBMITTER: Jessica Prenni 

PROVIDER: MTBLS2587 | MetaboLights | 2022-03-04

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS2587 Other
FILES Other
a_MTBLS2587_DART-MS_negative_metabolite_profiling.txt Txt
a_MTBLS2587_REIMS_positive_metabolite_profiling.txt Txt
files-all.json Other
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Publications

Evaluation of ambient mass spectrometry tools for assessing inherent postharvest pepper quality.

Mason Tyler J TJ   Bettenhausen Harmonie M HM   Chaparro Jacqueline M JM   Uchanski Mark E ME   Prenni Jessica E JE  

Horticulture research 20210701 1


Horticulturists are interested in evaluating how cultivar, environment, or production system inputs can affect postharvest quality. Ambient mass spectrometry approaches enable analysis of minimally processed samples under ambient conditions and offer an attractive high-throughput alternative for assessing quality characteristics in plant products. Here, we evaluate direct analysis in real time (DART-MS) mass spectrometry and rapid evaporative ionization-mass spectrometry (REIMS) to assess qualit  ...[more]

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