Dataset Information

Individual variability in human blood metabolites identifies age-related differences (30 persons, whole blood data)

ABSTRACT: Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences.

Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

INSTRUMENT(S): LTQ Orbitrap Classic (Thermo Scientific)

SUBMITTER: Romanas Chaleckis

PROVIDER: MTBLS265 | MetaboLights | 2016-03-10

REPOSITORIES: MetaboLights

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Publications

Individual variability in human blood metabolites identifies age-related differences.

Chaleckis Romanas R Murakami Itsuo I Takada Junko J Kondoh Hiroshi H Yanagida Mitsuhiro M

Proceedings of the National Academy of Sciences of the United States of America 20160328 16

Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which met ...[more]

PMID: 27036001

Similar Datasets

Project description:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. Validation of experimental procedures was performed as follows. First, we evaluated the contribution of sample handling to within-sample variation. The same blood sample preparation was injected 3x into the LC-MS at 80-min intervals. We thus obtained within-sample CVs (designated as CVwi), which were less than 0.1 in 107 of 126 compounds (85%). Only 10 compounds showed CVwi of 0.1-0.2, while 9 had CVwi >0.2. Second, we also examined sample-to-sample variation caused by sample preparation. Three samples were independently prepared from the same blood sample (one person), and CVs thus determined were designated as CVss. CVss values of HEPES and PIPES in the blood samples were very small (0.06~0.08 for HEPES and 0.04~0.08 for PIPES). The great majority (116/126=92%) of CVss were <0.3. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

Project description:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and life-style factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed non-targeted, quantitative metabolomics analysis in blood of 15 young (29±4 yr) and 15 elderly (81±7 yr) individuals. Coefficients of variation (CV=standard deviation/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found fourteen blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in anti-oxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. While our CV values are mostly consistent with those previously published, we here report novel CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4-2.5) are often modified. Compounds having low CV values such as ATP and glutathione may be related to various diseases as their concentrations are strictly controlled, and changes in them would compromise health. Thus human blood is a rich source of information about individual metabolic differences. Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. RBC data from all 30 subjects can be found under MTBLS267.

Project description:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. In human 24 hr metabolome observation (non-fasting) four volunteers were taken blood after overnight fast without breakfast at 9:00; 10:00, 13:00 and before lunch on the first day. Volunteers had lunches and dinners as usual on that day. On the second day after overnight fast the blood was sampled again at 9:00 at Kyoto university hospital laboratory. The great majority of metabolites hardly fluctuated (117 from 126 metabolites varied less than 2.5-fold on average in four volunteers). Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

Project description:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and life-style factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed non-targeted, quantitative metabolomics analysis in blood of 15 young (29±4 yr) and 15 elderly (81±7 yr) individuals. Coefficients of variation (CV=standard deviation/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found fourteen blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in anti-oxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. While our CV values are mostly consistent with those previously published, we here report novel CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4-2.5) are often modified. Compounds having low CV values such as ATP and glutathione may be related to various diseases as their concentrations are strictly controlled, and changes in them would compromise health. Thus human blood is a rich source of information about individual metabolic differences. Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma data from all 30 subjects can be found under MTBLS266.

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS265 Abstract:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS266 Abstract:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and life-style factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed non-targeted, quantitative metabolomics analysis in blood of 15 young (29±4 yr) and 15 elderly (81±7 yr) individuals. Coefficients of variation (CV=standard deviation/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found fourteen blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in anti-oxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. While our CV values are mostly consistent with those previously published, we here report novel CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4-2.5) are often modified. Compounds having low CV values such as ATP and glutathione may be related to various diseases as their concentrations are strictly controlled, and changes in them would compromise health. Thus human blood is a rich source of information about individual metabolic differences. Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. RBC data from all 30 subjects can be found under MTBLS267.

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS267 Abstract:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and life-style factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed non-targeted, quantitative metabolomics analysis in blood of 15 young (29±4 yr) and 15 elderly (81±7 yr) individuals. Coefficients of variation (CV=standard deviation/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found fourteen blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in anti-oxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. While our CV values are mostly consistent with those previously published, we here report novel CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4-2.5) are often modified. Compounds having low CV values such as ATP and glutathione may be related to various diseases as their concentrations are strictly controlled, and changes in them would compromise health. Thus human blood is a rich source of information about individual metabolic differences. Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma data from all 30 subjects can be found under MTBLS266.

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS264 Abstract:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. In human 24 hr metabolome observation (non-fasting) four volunteers were taken blood after overnight fast without breakfast at 9:00; 10:00, 13:00 and before lunch on the first day. Volunteers had lunches and dinners as usual on that day. On the second day after overnight fast the blood was sampled again at 9:00 at Kyoto university hospital laboratory. The great majority of metabolites hardly fluctuated (117 from 126 metabolites varied less than 2.5-fold on average in four volunteers). Data from three injections of the same sample and three samples prepared from the same donated blood are available under accession number MTBLS263. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

Project description:This data set is downloaded from MetaboLights (http://www.ebi.ac.uk/metabolights/) accession number MTBLS263 Abstract:Metabolites present in human blood document individual physiological states influenced by genetic, epigenetic, and lifestyle factors. Using high-resolution liquid chromatography-mass spectrometry (LC-MS), we performed nontargeted, quantitative metabolomics analysis in blood of 15 young (29 ± 4 y of age) and 15 elderly (81 ± 7 y of age) individuals. Coefficients of variation (CV = SD/mean) were obtained for 126 blood metabolites of all 30 donors. Fifty-five RBC-enriched metabolites, for which metabolomics studies have been scarce, are highlighted here. We found 14 blood compounds that show remarkable age-related increases or decreases; they include 1,5-anhydroglucitol, dimethyl-guanosine, acetyl-carnosine, carnosine, ophthalmic acid, UDP-acetyl-glucosamine, N-acetyl-arginine, N6-acetyl-lysine, pantothenate, citrulline, leucine, isoleucine, NAD+, and NADP+. Six of them are RBC-enriched, suggesting that RBC metabolomics is highly valuable for human aging research. Age differences are partly explained by a decrease in antioxidant production or increasing inefficiency of urea metabolism among the elderly. Pearson’s coefficients demonstrated that some age-related compounds are correlated, suggesting that aging affects them concomitantly. Although our CV values are mostly consistent with those CVs previously published, we here report previously unidentified CVs of 51 blood compounds. Compounds having moderate to high CV values (0.4–2.5) are often modified. Compounds having low CV values, such as ATP and glutathione, may be related to various diseases because their concentrations are strictly controlled, and changes in them would compromise health. Thus, human blood is a rich source of information about individual metabolic differences. Validation of experimental procedures was performed as follows. First, we evaluated the contribution of sample handling to within-sample variation. The same blood sample preparation was injected 3x into the LC-MS at 80-min intervals. We thus obtained within-sample CVs (designated as CVwi), which were less than 0.1 in 107 of 126 compounds (85%). Only 10 compounds showed CVwi of 0.1-0.2, while 9 had CVwi >0.2. Second, we also examined sample-to-sample variation caused by sample preparation. Three samples were independently prepared from the same blood sample (one person), and CVs thus determined were designated as CVss. CVss values of HEPES and PIPES in the blood samples were very small (0.06~0.08 for HEPES and 0.04~0.08 for PIPES). The great majority (116/126=92%) of CVss were <0.3. Blood samples drawn from four volunteers four times within 24 h are available under accession number MTBLS264. Whole blood metabolomic data from all 30 subjects are available under accession number MTBLS265. Plasma and RBC data from all 30 subjects can be found under MTBLS266 and MTBLS267, respectively.

			Action	DRS
	MTBLS265	Other
	FILES	Other
	a_MTBLS265_NEG_mass_spectrometry.txt	Txt
	a_MTBLS265_POS_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt