Dataset Information

Analysis of colorectal adenocarcinoma tissue samples by DESI mass spectrometry imaging

ABSTRACT: Ex vivo colorectal adenocarcinoma samples were analysed by desorption electrospray ionisation using an Orbitrap mass spectrometer. The samples were analysed in negative mode over the m/z range 600-1000. Some of the samples presented in this dataset were analysed as part of [1]. The study makes available imzML files of profile and centroid mode data, as well as low- and high- resolution optical image files of H&E-stained sections. These files can be found in the zip file named after each tissue section.

Ref:
[1] Gerbig S, Golf O, Balog J, Denes J, Baranyai Z, Zarand A, Raso E, Timar J, Takats Z. Analysis of colorectal adenocarcinoma tissue by desorption electrospray ionization mass spectrometric imaging. Anal Bioanal Chem. 2012 Jun;403(8):2315-25. doi:10.1007/s00216-012-5841-x. PMID:22447214

INSTRUMENT(S): Exactive

SUBMITTER: James S McKenzie

PROVIDER: MTBLS289 | MetaboLights | 2016-04-28

REPOSITORIES: MetaboLights

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Analysis of colorectal adenocarcinoma tissue by desorption electrospray ionization mass spectrometric imaging.

Gerbig Stefanie S Golf Ottmar O Balog Julia J Denes Julia J Baranyai Zsolt Z Zarand Attila A Raso Erzsebet E Timar Jozsef J Takats Zoltan Z

Analytical and bioanalytical chemistry 20120325 8

Negative ion desorption electrospray ionization (DESI) was used for the analysis of an ex vivo tissue sample set comprising primary colorectal adenocarcinoma samples and colorectal adenocarcinoma liver metastasis samples. Frozen sections (12 μm thick) were analyzed by means of DESI imaging mass spectrometry (IMS) with spatial resolution of 100 μm using a computer-controlled DESI imaging stage mounted on a high resolution Orbitrap mass spectrometer. DESI-IMS data were found to predominantly featu ...[more]

PMID: 22447214

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			Action	DRS
	MTBLS289	Other
	FILES	Other
	a_MTBLS289_ml-icl-colo-new_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	s_MTBLS289.txt	Txt