Dataset Information

Metabolomics dataset of PPAR-pan treated rat liver (acyl-carnitine and aqueous metabolite assays)

ABSTRACT:

This article contains mass spectrometry (MS) data investigating small molecule changes as an effect of a triple peroxisome proliferator-activated receptor (PPAR-pan) agonist GW625019 in the liver as described in the manuscript “PPAR-pan Activation Induces Hepatic Oxidative Stress and Lipidomic Remodelling“ [1]. Samples were analysed using liquid chromatography-mass spectrometry (LC-MS) to measure intact lipids, carnitines and selected aqueous metabolites and eicosanoids. Data files comprise of Excel (Microsoft, WA, USA) spreadsheets of identified metabolites and there area ratio values for total fatty acids, carnitines, aqueous metabolites, and eicosanoids where the intensity of the analytes were normalised to the intensity of the internal standard. In the case of open profiling intact lipid data, the Excel file contains area ratio values of retention time and mass to charge ratio pairs; again, the area ratio values were calculated by normalising to the intensity of the internal standard. It should be noted that several metabolic changes are potentially indirect (secondary, tertiary and ensuing changes).

The protocols and data of the carnitine and targeted aqueous metabolites are included in current study MTBLS303.

The protocols and data of the eicosanoids are reported in the study MTBLS278.

INSTRUMENT(S): API 5500 QTRAP (AB Sciex)

SUBMITTER: Zsuzsanna Ament

PROVIDER: MTBLS303 | MetaboLights | 2016-05-13

REPOSITORIES: MetaboLights

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Publications

PPAR-pan activation induces hepatic oxidative stress and lipidomic remodelling.

Ament Zsuzsanna Z West James A JA Stanley Elizabeth E Ashmore Tom T Roberts Lee D LD Wright Jayne J Nicholls Andrew W AW Griffin Julian L JL

Free radical biology & medicine 20151130

The peroxisome proliferator-activated receptors (PPARs) are ligand activated nuclear receptors that regulate cellular homoeostasis and metabolism. PPARs control the expression of genes involved in fatty-acid and lipid metabolism. Despite evidence showing beneficial effects of their activation in the treatment of metabolic diseases, particularly dyslipidaemias and type 2 diabetes, PPAR agonists have also been associated with a variety of side effects and adverse pathological changes. Agonists hav ...[more]

PMID: 26654758

Publication: 1/2

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			Action	DRS
	MTBLS303	Other
	FILES	Other
	a_MTBLS303_Ppar_pan_acyl_carnitine.txt	Txt
	a_MTBLS303_Ppar_pan_aqueous_metabolites.txt	Txt
	i_Investigation.txt	Txt