Dataset Information

Evidence of a DHA signature in the lipidome and metabolome of human hepatocytes

ABSTRACT: Cell supplementation with bioactive molecules often causes a perturbation in the whole intracellular environment. Omics techniques can be applied for the assessment of this perturbation. In this study, the overall effect of docosahexaenoic acid (DHA) supplementation on cultured human hepatocyte lipidome and metabolome has been investigated using NMR in combination with traditional techniques. The effect of two additional bioactives sharing with DHA the lipid lowering effect - propionic acid (PRO) and protocatechuic acid (PCA) - has been also evaluated, also considering possible synergism. NMR analysis of the cell lipid extracts showed that DHA supplementation, alone or in combination with PCA or PRO, strongly altered the cell lipid profile. The perfect discrimination between cells receiving DHA (alone or in combination) and the other cells reinforced the idea of a global rearrangement of the lipid environment induced by DHA. Notably, GC and fluorimetric analyses totally confirmed the strong discrimination obtained by NMR. The DHA signature was evidenced not only in the cell lipidome, but also in the metabolome. Results herein reported indicate that NMR, combined with other techniques, represents a fundamental approach while studying the effect of the supplementation of bioactives, particularly in the case of molecules having a broad spectrum of mechanisms of action.

INSTRUMENT(S): Bruker

SUBMITTER: veronica ghini

PROVIDER: MTBLS419 | MetaboLights | 2017-03-02

REPOSITORIES: MetaboLights

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Dataset's files

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			Action	DRS
	MTBLS419	Other
	FILES	Other
	a_MTBLS419_pathway_metabolite_profiling_NMR_spectroscopy.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS419_pathway_metabolite_profiling_NMR_spectroscopy_v2_maf.tsv	Tabular

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Evidence of a DHA Signature in the Lipidome and Metabolome of Human Hepatocytes.

Ghini Veronica V Di Nunzio Mattia M Tenori Leonardo L Valli Veronica V Danesi Francesca F Capozzi Francesco F Luchinat Claudio C Bordoni Alessandra A

International journal of molecular sciences 20170208 2

Cell supplementation with bioactive molecules often causes a perturbation in the whole intracellular environment. Omics techniques can be applied for the assessment of this perturbation. In this study, the overall effect of docosahexaenoic acid (DHA) supplementation on cultured human hepatocyte lipidome and metabolome has been investigated using nuclear magnetic resonance (NMR) in combination with traditional techniques. The effect of two additional bioactives sharing with DHA the lipid-lowering ...[more]

PMID: 28208746

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