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Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions

ABSTRACT: Metabolomics is a modern tool that aids in our understanding of the molecular changes in organisms. Archaeological science is a branch of archaeology that explores different archaeological materials using modern analytical tools. Human osteoarchaeological material are a frequent finding in archaeological contexts and have the potential to offer information about previous human populations, which can be illuminating about our current condition. Using a set of samples comprising different skeletal elements and bone structures, here we explore for the first time the possibility of extracting metabolites from osteoarchaeological material. Here, a protocol for extraction and measurement of extracted polar and less-polar/apolar metabolites by ultra-high performance liquid chromatography hyphenated to high resolution mass spectrometry is presented to measure the molecules separated after a reversed phase and hydrophilic interaction liquid chromatography column. Molecular information was obtained, showing that osteoarchaeological material is a viable source of molecular information for metabolomic studies.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - positive - reverse phase, Liquid Chromatography MS - negative - hilic

PROVIDER: MTBLS4437 | MetaboLights | 2022-07-04

REPOSITORIES: MetaboLights

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Examination of human osteoarchaeological remains as a feasible source of polar and apolar metabolites to study past conditions.

Badillo-Sanchez Diego D Ruber Maria Serrano MS Davies-Barrett Anna M AM Sandhu Jatinderpal K JK Jones Donald J L DJL Hansen Martin M Inskip Sarah A SA

Scientific reports 20230113 1

Metabolomics is a modern tool that aids in our understanding of the molecular changes in organisms. Archaeological science is a branch of archaeology that explores different archaeological materials using modern analytical tools. Human osteoarchaeological material are a frequent finding in archaeological contexts and have the potential to offer information about previous human populations, which can be illuminating about our current condition. Using a set of samples comprising different skeletal ...[more]

PMID: 36639564

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			Action	DRS
	117_C18_Neg.raw.zip	Raw
	117_C18_Pos.raw.zip	Raw
	117_HILIC_Neg.raw.zip	Raw
	117_HILIC_Pos.raw.zip	Raw
	118_C18_Neg.raw.zip	Raw