Dataset Information

Automated high-throughput biological sex identification from archaeological human dental enamel using targeted proteomics

ABSTRACT: Biological sex is a key information for archaeological and forensic studies, which can be assessed in enamel using proteomics. However, the lack of a standard approach for fast and accurate sex identification limits the reach of proteomics applications. Here, we introduce a targeted proteomics-based workflow that builds on a rapid, non-destructive, sensitive, and quantitative mass spectrometry (MS) strategy for the determination of biological sex from human dental enamel, including a R-Shiny interface for the data analysis. The method was first developed and optimised using modern teeth material and then validated on an independent set of modern deciduous teeth of known sex. The assay was then applied to archaeological material and demonstrated unprecedented specificity and scalability for molecular sex inference, with the possibility of analysing > 300 individuals in less than two days of MS measurement time, and with subsequent confident sex assignment in less than a day of data analysis.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Enamel, Tooth Enamel

SUBMITTER: Claire Koenig

LAB HEAD: Jesper V. Olsen

PROVIDER: PXD049326 | Pride | 2024-10-17

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20210527_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_enamel_1918.raw	Raw
	20210527_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_enamel_1920.raw	Raw
	20210527_EXPL2_nLC11_MEM_collab_77min_DDA_Ancient_enamel_1922.raw	Raw
	20211124_EXPL5_nLC3_MEM_collab_77min_DDA_Ancient_enamel_1965.raw	Raw
	20211124_EXPL5_nLC3_MEM_collab_77min_DDA_Ancient_enamel_1966.raw	Raw

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Publications

Automated High-Throughput Biological Sex Identification from Archeological Human Dental Enamel Using Targeted Proteomics.

Koenig Claire C Bortel Patricia P Paterson Ryan S RS Rendl Barbara B Madupe Palesa P PP Troché Gaudry B GB Hermann Nuno Vibe NV Martínez de Pinillos Marina M Martinón-Torres María M Mularczyk Sandra S Schjellerup Jørkov Marie Louise ML Gerner Christopher C Kanz Fabian F Martinez-Val Ana A Cappellini Enrico E Olsen Jesper V JV

Journal of proteome research 20240926 11

Biological sex is key information for archeological and forensic studies, which can be determined by proteomics. However, the lack of a standardized approach for fast and accurate sex identification currently limits the reach of proteomics applications. Here, we introduce a streamlined mass spectrometry (MS)-based workflow for the determination of biological sex using human dental enamel. Our approach builds on a minimally invasive sampling strategy by acid etching, a rapid online liquid chromat ...[more]

PMID: 39324540

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