Dataset Information

The Green Tea Modulates Large Intestinal Microbiome and Exo/Endogenous Metabolome altered through Chronic UVB-exposure (Endogenous assays)

ABSTRACT: The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-exposed mice groups (UVB), class Bacilli and order Bifidobacteriales were observed as discriminant taxa with decreased lysophospholipid levels compared to the unexposed mice groups subjected to normal diet (NOR). Conversely, in GTS fed UVB-exposed mice (U+GTS), the gut-microbiome diversity was greatly enhanced with enrichment in the classes, Clostridia and Erysipelotrichia, as well as genera, Allobaculum and Lachnoclostridium. Additionally, the gut endogenous metabolomes changed with an increase in amino acids, fatty acids, lipids, and bile acids contents coupled with a decrease in nucleobases and carbohydrate levels. The altered metabolomes exhibited high correlations with GTS enriched intestinal microflora. Intriguingly, the various conjugates of green tea catechins viz., sulfated, glucuronided, and methylated ones including their exogenous derivatives were detected from large intestinal contents and liver samples. Hence, we conjecture that the metabolic conversions for the molecular components in GTS strongly influenced the gut micro-environment in UVB-exposed mice groups, ergo modulate their gut-microbiome as well as exo/endogenous metabolomes.

INSTRUMENT(S): Liquid Chromatography MS - Negative (LC-MS (Negative)), Gas Chromatography MS - Positive (GC-MS (Positive))

SUBMITTER: Eun Sung Jung

PROVIDER: MTBLS527 | MetaboLights | 2018-09-14

REPOSITORIES: MetaboLights

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The green tea modulates large intestinal microbiome and exo/endogenous metabolome altered through chronic UVB-exposure.

Jung Eun Sung ES Park Hye Min HM Hyun Seung Min SM Shon Jong Cheol JC Singh Digar D Liu Kwang-Hyeon KH Whon Tae Woong TW Bae Jin-Woo JW Hwang Jae Sung JS Lee Choong Hwan CH

PloS one 20171108 11

The attenuating effects of green tea supplements (GTS) against the ultraviolet (UV) radiation induced skin damages are distinguished. However, the concomitant effects of GTS on the large intestinal microbiomes and associated metabolomes are largely unclear. Herein, we performed an integrated microbiome-metabolome analysis to uncover the esoteric links between gut microbiome and exo/endogenous metabolome maneuvered in the large intestine of UVB-exposed mice subjected to dietary GTS. In UVB-expose ...[more]

PMID: 29117187

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			Action	DRS
	MTBLS527	Other
	FILES	Other
	a_MTBLS527_GC_mass_spectrometry.txt	Txt
	a_MTBLS527_UPLC_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt