Dataset Information

Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance

ABSTRACT: Commensal intestinal bacteria play key roles in regulating cells mediating immune tolerance; however, bacterial strains and related metabolites directly involved in this regulation are largely unknown. Here, using a mouse model of dextran sulfate sodium (DSS)-induced colitis combined with different antibiotic treatment, Enterobacter ludwigii, abundant in microbiota of mice treated with metronidazole, was screened out to have prophylactic and therapeutic effects on DSS-induced colitis with or without the presence of complex intestinal bacteria. E. ludwigii was demonstrated to induce CD103+DC and Treg-mediated immune tolerance for colitis remission using in vitro and in vivo experiments. Moreover, choline, one of E. ludwigii metabolites, was identified to increase DCs’ immune tolerance to promote Treg differentiation. E. ludwigii was found to induce DCs’ immune tolerance ability for Treg differentiation through choline and α7nAChR-mediated RA and TGF-β upregulation, resulting in protecting mice against DSS-induced colitis. This study provides potential therapeutic approaches for IBD.

INSTRUMENT(S): Liquid Chromatography MS - negative - reverse phase, Liquid Chromatography MS - positive - reverse phase

SUBMITTER: qianqian li

PROVIDER: MTBLS5585 | MetaboLights | 2024-08-05

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS5585	Other
	FILES	Other
	a_MTBLS5585_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS5585_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt

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Publications

Enterobacter ludwigii protects DSS-induced colitis through choline-mediated immune tolerance.

Li Qianqian Q Sun Xuan X Yu Kaiyuan K Lv Junqiang J Miao Chunhui C Yang Jianming J Wang Song S Fu Zheng Z Sun Yamin Y Zhang Hong H Zhang Zhi-Song ZS Keller Evan T ET Yao Zhi Z Wang Quan Q

Cell reports 20220801 9

Commensal intestinal bacteria play key roles in regulating host immune tolerance; however, bacterial strains and related metabolites directly involved in this regulation are largely unknown. Here, using a mouse model of dextran sulfate sodium (DSS)-induced colitis combined with different antibiotic treatment, Enterobacter ludwigii, abundant in microbiota of mice treated with metronidazole, is screened out to have prophylactic and therapeutic effects on DSS-induced colitis with or without the pre ...[more]

PMID: 36044853

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