Dataset Information

Diagnostic metabolite biomarkers of chronic typhoid carriage

ABSTRACT: Background: Salmonella Typhi and Salmonella Paratyphi A are the agents of enteric (typhoid) fever; both can establish chronic carriage in the gallbladder. Chronic Salmonella carriers are typically asymptomatic, intermittently shedding bacteria in the feces, and contributing to disease transmission. Detecting chronic carriers is of public health relevance in areas where enteric fever is endemic, but there are no routinely used methods for prospectively identifying those carrying Salmonella in their gallbladder. Methodology/Principal Findings: Here we aimed to identify biomarkers of Salmonella carriage using metabolite profiling. We performed metabolite profiling on plasma from Nepali patients undergoing cholecystectomy with confirmed S. Typhi or S. Paratyphi A gallbladder carriage (and non-carriage controls) using two-dimensional gas chromatography coupled with time-of-flight mass spectrometry (GCxGC-TOFMS) and supervised pattern recognition modeling. We were able to significantly discriminate Salmonella carriage samples from non-carriage control samples. We were also able to detect differential signatures between S. Typhi and S. Paratyphi A carriers. We additionally compared carriage metabolite profiles with profiles generated during acute infection; these data revealed substantial heterogeneity between metabolites associated with acute enteric fever and chronic carriage. Lastly, we found that Salmonella carriers could be significantly distinguished from non-carriage controls using only five metabolites, indicating the potential of these metabolites as diagnostic markers for detecting chronic Salmonella carriers. Conclusions/Significance: Our novel approach has highlighted the potential of using metabolomics to search for diagnostic markers of chronic Salmonella carriage. We suggest further epidemiological investigations of these potential biomarkers in alternative endemic enteric fever settings.

INSTRUMENT(S): Pegasus 4D GCxGC-TOFMS

SUBMITTER: Elin Näsström

PROVIDER: MTBLS579 | MetaboLights | 2018-01-17

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS579	Other
	FILES	Other
	a_MTBLS579_typhoid_carriage_metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS579_typhoid_carriage_metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

Diagnostic metabolite biomarkers of chronic typhoid carriage.

Näsström Elin E Jonsson Pär P Johansson Anders A Dongol Sabina S Karkey Abhilasha A Basnyat Buddha B Tran Vu Thieu Nga N Trinh Van Tan T Thwaites Guy E GE Antti Henrik H Baker Stephen S

PLoS neglected tropical diseases 20180126 1

<h4>Background</h4>Salmonella Typhi and Salmonella Paratyphi A are the agents of enteric (typhoid) fever; both can establish chronic carriage in the gallbladder. Chronic Salmonella carriers are typically asymptomatic, intermittently shedding bacteria in the feces, and contributing to disease transmission. Detecting chronic carriers is of public health relevance in areas where enteric fever is endemic, but there are no routinely used methods for prospectively identifying those carrying Salmonella ...[more]

PMID: 29373578

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