Metabolomics

Dataset Information

0

Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry


ABSTRACT: Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the highest signal intensity; 17 rhamnolipids and 18 quorum sensing molecules were detected, demonstrating that REIMS has potential for the study of virulence-related metabolites. P. aeruginosa isolates obtained from respiratory samples showed a higher diversity, which was attributed to the chronic nature of most respiratory infections. The analytical sensitivity of REIMS allowed the detection of a metabolome that could be used to classify individual P. aeruginosa isolates after repeated culturing with 81% accuracy, and an average 83% concordance with multilocus sequence typing. This study underpins the capacities of REIMS as a tool with clinical applications, such as metabolic phenotyping of the important CF pathogen P. aeruginosa, and highlights the potential of metabolic fingerprinting for fine scale characterisation at a sub-species level.

INSTRUMENT(S): Direct infusion MS - negative

SUBMITTER: Simon Cameron 

PROVIDER: MTBLS644 | MetaboLights | 2022-01-26

REPOSITORIES: MetaboLights

Dataset's files

Source:
Action DRS
MTBLS644 Other
FILES Other
a_MTBLS644_DI-MS_negative__metabolite_profiling.txt Txt
files-all.json Other
i_Investigation.txt Txt
Items per page:
1 - 5 of 7
altmetric image

Publications

Metabolic Phenotyping and Strain Characterisation of Pseudomonas aeruginosa Isolates from Cystic Fibrosis Patients Using Rapid Evaporative Ionisation Mass Spectrometry.

Bardin Emmanuelle E EE   Cameron Simon J S SJS   Perdones-Montero Alvaro A   Hardiman Kate K   Bolt Frances F   Alton Eric W F W EWFW   Bush Andrew A   Davies Jane C JC   Takáts Zoltan Z  

Scientific reports 20180719 1


Rapid evaporative ionisation mass spectrometry (REIMS) is a novel technique for the real-time analysis of biological material. It works by conducting an electrical current through a sample, causing it to rapidly heat and evaporate, with the analyte containing vapour channelled to a mass spectrometer. It was used to characterise the metabolome of 45 Pseudomonas aeruginosa (P. aeruginosa) isolates from cystic fibrosis (CF) patients and compared to 80 non-CF P. aeruginosa. Phospholipids gave the hi  ...[more]

Similar Datasets

2011-08-05 | E-GEOD-31227 | biostudies-arrayexpress
2011-12-31 | GSE33275 | GEO
2009-02-05 | GSE10304 | GEO
2008-12-05 | GSE6122 | GEO
2011-12-31 | E-GEOD-33275 | biostudies-arrayexpress
2011-08-06 | GSE31227 | GEO
2020-05-31 | ST001414 | MetabolomicsWorkbench
2022-08-24 | GSE209539 | GEO
2022-03-18 | GSE198534 | GEO
2022-03-18 | GSE198719 | GEO