Dataset Information

Gut microbiota-driven regulation of queen bee ovarian metabolism

ABSTRACT:

With the global prevalence of Varroa mites, more and more beekeepers resort to confining the queen bee in a queen cage to control mite infestation or to breed superior and robust queen bees. However, the impact of such practices on the queen bee remains largely unknown. Therefore, we subjected the queen bees to a 21-d egg-laying restriction treatment (from the egg stage to the emergence of adult worker bees) and analyzed the queen bees' ovarian metabolites and gut microbiota after 21 d, aiming to assess the queen bee's quality and assist beekeepers in better hive management. Our findings revealed a significant reduction in the relative expression levels of Vg and Hex110 genes in the ovaries of egg-laying-restricted queen bees compared to unrestricted egg-laying queens. The diversity of gut microbiota in the queen bee exhibited a notable decrease, accompanied by corresponding changes in the core bacterial species of the microbial community. Following egg-laying restriction, the activity of the queen bee's ovaries decreased, while the metabolism of glycerophospholipids remained or stored more lipid molecules, awaiting environmental changes for the queen bee to resume egg-laying promptly. Furthermore, we observed that Bombella in the queen bee's gut may regulate the queen's ovarian metabolism through tryptophan metabolism. These findings provide novel insights into the interplay among queen egg-laying, gut microbiota, and ovarian metabolism.

INSTRUMENT(S): Liquid Chromatography MS - positive - hilic, Liquid Chromatography MS - negative

SUBMITTER: Wanli Li

PROVIDER: MTBLS7576 | MetaboLights | 2023-09-11

REPOSITORIES: MetaboLights

ACCESS DATA

Dataset's files

Source:

			Action	DRS
	MTBLS7576	Other
	FILES	Other
	a_MTBLS7576_LC-MS_negative_reverse-phase_metabolite_profiling.txt	Txt
	a_MTBLS7576_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	files-all.json	Other

Items per page:

1 - 5 of 9

Publications

Gut microbiota-driven regulation of queen bee ovarian metabolism.

Li Wan-Li WL Huang Qi Q Li Jia-Li JL Wu Ping P Wei Bangrong B Li Xi-Jie XJ Tang Qi-He QH Dong Zhi-Xiang ZX Xiong Jian J Tang Hong H Zhang Jun J Zhao Chong-Hui CH Cao Zhe Z Chen Yuan Y Zhao Wen-Zheng WZ Wang Kai K Guo Jun J

Microbiology spectrum 20230926

With the global prevalence of <i>Varroa</i> mites, more and more beekeepers resort to confining the queen bee in a queen cage to control mite infestation or to breed superior and robust queen bees. However, the impact of such practices on the queen bee remains largely unknown. Therefore, we subjected the queen bees to a 21-day egg-laying restriction treatment (from the egg stage to the emergence of adult worker bees) and analyzed the queen bees' ovarian metabolites and gut microbiota after 21 da ...[more]

PMID: 37750696

Similar Datasets

Project description:Soybean is one of the most important sources of food, protein, and oil in the world. Reductions in grain number and quality are caused by different biotic stresses. One of the most common is the phytophagous mite Tetranychus urticae Koch (Acari: Tetranychidae), which inhibits plant growth and grain production. The identification of plant responses to early and late T. urticae infestation is important for a better understanding of the mite-plant interaction. We therefore aimed to evaluate the physiological and molecular responses of soybean plants to mite infestation for 5 and 21 days. Visual and microscopic symptoms of leaf damage, H2O2 accumulation, and lipid peroxidation increased consistently throughout the infestation period, while shoot length/dry weight, chlorophyll level, and number of days to reach specific developmental stages were negatively affected by T. urticae infestation. Using proteomic analysis, we identified 185 and 266 differentially abundant proteins after early (5 days) and late (21 days) mite infestation, respectively, which suggests a complex remodeling of diverse metabolic pathways. GO, KEGG, and protein-protein interaction analyses indicated that photorespiration, chlorophyll synthesis, amino acid metabolism, Krebs cycle/energy production, mitochondrial translation, nucleotide salvage, PS II assembly, and reductive pentose-P cycle are all impacted after both early and late infestation. Specific metabolic pathways modified only after early infestation include cell wall modification, cytoskeleton composition, cell division, and lysine/histidine metabolism, while JA biosynthesis, antioxidant system, S-adenosyl methionine cycle, PS II repair, cysteine/methionine/glutathione/ascorbate/-linolenic acid/selenocompound metabolism, arginine biosynthesis, and proteasome are modified only after late infestation. These differentially abundant proteins can be used as biotechnological tools in future breeding programs aiming at increased resistance to mite infestation.