Unravelling altered RNA metabolism in pancreatic cancer cells by liquid-chromatography coupling to ion mobility mass spectrometry

ABSTRACT: Ion mobility coupling to mass spectrometry facilitates an increase of resolution and chromatographic peak-capacity. Further coupling to liquid chromatography results in multi-dimensional analytical methods, especially suitable for complex matrices with structurally similar compounds. Modified nucleosides are a large group of very similar members and are linked to aberrant proliferation. Contiguous to basal production under physiological conditions, they are increasingly excreted by transformed cells and subsequently discussed as putative biomarkers for various cancer types. Therefore, they are discussed as putative biomarkers for various cancer types. Here we report a method for modified nucleosides covering 37 species. We determined collisional cross-sections with high reproducibility from pure analytical standards. We applied an optimized phenyl-boronic acid solid-phase extraction on media obtained from four different pancreatic cancer cell lines. Our analysis could discriminate different sub-types of pancreatic cancer cell lines. Importantly, they clearly separated from a pancreatic control cell line and blank medium. m1A, m27G and Asm were the most important features discriminating cancer cell lines derived from well differentiated and poorly differentiated cancers. Eventually, we suggest the analytical method reported here for future tumor-marker identification studies.

INSTRUMENT(S): SYNAPT G2 HDMS (Waters)

SUBMITTER: Simon Lagies 

PROVIDER: MTBLS812 | MetaboLights | 2019-05-16

REPOSITORIES: MetaboLights