Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry
Ontology highlight
ABSTRACT:
Solid phase extraction is reported in the current study MTBLS849.
Method development is reported in MTBLS826.
The effect of salt and water on metabolite detection is reported in MTBLS839.
Cell culture sampling is reported in MTBLS843.
Environmental sampling is reported in MTBLS844.
Method quantification is reported in MTBLS848.
Linked Studies: MTBLS826 MTBLS839 MTBLS843 MTBLS844 MTBLS848
INSTRUMENT(S): Gas Chromatography MS - Positive (GC-MS (Positive))
SUBMITTER: Manuel Liebeke
PROVIDER: MTBLS849 | MetaboLights | 2019-11-15
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| MTBLS849 | Other | |||
| FILES | Other | |||
| a_MTBLS849_metabolite_profiling_mass_spectrometry.txt | Txt | |||
| i_Investigation.txt | Txt | |||
| s_MTBLS849.txt | Txt |
Items per page: 5 1 - 5 of 5 |
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Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method quantification is reported in the current study MTBLS848.</br> Method development is reported in MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
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Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method development is reported in the current study MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Method quantification is reported in MTBLS848.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
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Project description:<p>One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </p><p><br></p><p>Cell culture sampling is reported in the current study.</p><p>Method development is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS826" target="_blank">MTBLS826</a></p><p>The effect of salt and water on metabolite detection is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS839" target="_blank">MTBLS839</a></p><p>Environmental sampling is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS844" target="_blank">MTBLS844</a></p><p>Method quantification is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS848" target="_blank">MTBLS848</a></p><p>Solid phase extraction is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS849" target="_blank">MTBLS849</a></p>
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Project description:The characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyse the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signalling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry. </br></br> The General LC-MS assay is reported in the current study MTBLS629. </br> The Lipidomic LC-MS assay is reported in MTBLS631. </br> The CE-MS assay is reported in MTBLS633. </br> The GC-MS assay is reported in MTBLS634. </br></br> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS631' target='_blank'><span class='label label-success'>MTBLS631</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS633' target='_blank'><span class='label label-success'>MTBLS633</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS634' target='_blank'><span class='label label-success'>MTBLS634</span></a>
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