Marine metabolomics: measurement of metabolites in seawater by gas chromatography mass spectrometry (Cell culture sampling)
Ontology highlight
ABSTRACT: One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. Cell culture sampling is reported in the current study. Method development is reported in MTBLS826 The effect of salt and water on metabolite detection is reported in MTBLS839 Environmental sampling is reported in MTBLS844 Method quantification is reported in MTBLS848 Solid phase extraction is reported in MTBLS849
INSTRUMENT(S): Gas Chromatography MS - Positive (GC-MS (Positive))
SUBMITTER: Manuel Liebeke
PROVIDER: MTBLS843 | MetaboLights | 2019-11-15
REPOSITORIES: MetaboLights
Dataset's files
| Action | DRS | |||
|---|---|---|---|---|
| MTBLS843 | Other | |||
| FILES | Other | |||
| a_MTBLS843_metabolite_profiling_mass_spectrometry.txt | Txt | |||
| i_Investigation.txt | Txt | |||
| m_MTBLS843_metabolite_profiling_mass_spectrometry_v2_maf.tsv | Tabular |
Items per page: 5 1 - 5 of 7 |
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Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Solid phase extraction is reported in the current study MTBLS849.</br> Method development is reported in MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Method quantification is reported in MTBLS848.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a>
2019-11-15 | MTBLS849 | MetaboLights
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> The effect of salt and water on metabolite detection is reported in the current study MTBLS839.</br> Method development is reported in MTBLS826.</br> Cell culture sampling is reported MTBLS843.</br> Environmental sampling is reported MTBLS844.</br> Method quantification is reported in MTBLS848.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
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Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method quantification is reported in the current study MTBLS848.</br> Method development is reported in MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS826' target='_blank'><span class='label label-success'>MTBLS826</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
2019-11-15 | MTBLS848 | MetaboLights
Project description:One of the major bottlenecks in describing marine ecosystems is characterizing the metabolome of seawater, as salt prevents metabolite analysis. We present SeaMet, a method that can detect hundreds of metabolites in less than one ml of seawater and quantifies them down to nano-molar levels using gas chromatography-mass spectrometry. Our method provides a major advance in marine metabolomics by enabling the unbiased analysis of primary metabolites across saltwater habitats. </br></br> Method development is reported in the current study MTBLS826.</br> The effect of salt and water on metabolite detection is reported in MTBLS839.</br> Cell culture sampling is reported in MTBLS843.</br> Environmental sampling is reported in MTBLS844.</br> Method quantification is reported in MTBLS848.</br> Solid phase extraction is reported in MTBLS849.</br> </br><br/> Linked Studies: <a href='https://www.ebi.ac.uk/metabolights/MTBLS839' target='_blank'><span class='label label-success'>MTBLS839</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS843' target='_blank'><span class='label label-success'>MTBLS843</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS844' target='_blank'><span class='label label-success'>MTBLS844</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS848' target='_blank'><span class='label label-success'>MTBLS848</span></a> <a href='https://www.ebi.ac.uk/metabolights/MTBLS849' target='_blank'><span class='label label-success'>MTBLS849</span></a>
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2016-01-05 | PXD001491 | Pride
Project description:<p>Women have a lower incidence of colorectal cancer (CRC) than men, however, they have a higher prevalence of right-sided colon cancer (RCC). This is of concern as patients with RCC have the poorest clinical outcomes amongst CRC patients. Aberrant metabolism is a feature of CRC, but it is not known if sex-differences exist. Metabolomics of patient colon tissues (n=236) revealed widespread sex-differences in metabolism depending on tumor location and stage. Tumors from women with RCC were nutrient-deplete, showing enhanced energy production towards asparagine synthesis, and an increase in amino acid uptake. Gene expression data revealed an association between high asparagine synthase expression and poorer overall survival in women with CRC. This is the first study to show sex-differences in tumor tissue metabolism for CRC patients revealing a nutrient-deplete subtype in women with RCC. The results therefore have implications for tumor progression and therapeutic response in women with RCC.</p><p><br></p><p>Linked Studies:</p><p>UPLC-MS HILIC POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1122" target="_blank">MTBLS1122</a></p><p>UPLC-MS HILIC NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1124" target="_blank">MTBLS1124</a></p><p>UPLC-MS RP POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1129" target="_blank">MTBLS1129</a></p><p>UPLC-MS RP NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1130" target="_blank">MTBLS1130</a></p>
2020-08-01 | MSV000085874 | GNPS
Project description:<p>Women have a lower incidence of colorectal cancer (CRC) than men, however, they have a higher prevalence of right-sided colon cancer (RCC). This is of concern as patients with RCC have the poorest clinical outcomes amongst CRC patients. Aberrant metabolism is a feature of CRC, but it is not known if sex-differences exist. Metabolomics of patient colon tissues (n=236) revealed widespread sex-differences in metabolism depending on tumor location and stage. Tumors from women with RCC were nutrient-deplete, showing enhanced energy production towards asparagine synthesis, and an increase in amino acid uptake. Gene expression data revealed an association between high asparagine synthase expression and poorer overall survival in women with CRC. This is the first study to show sex-differences in tumor tissue metabolism for CRC patients revealing a nutrient-deplete subtype in women with RCC. The results therefore have implications for tumor progression and therapeutic response in women with RCC.</p><p><br></p><p>Linked Studies:</p><p>UPLC-MS HILIC POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1122" target="_blank">MTBLS1122</a></p><p>UPLC-MS HILIC NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1124" target="_blank">MTBLS1124</a></p><p>UPLC-MS RP POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1129" target="_blank">MTBLS1129</a></p><p>UPLC-MS RP NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1130" target="_blank">MTBLS1130</a></p>
2020-08-01 | MSV000085884 | GNPS
Project description:<p>Women have a lower incidence of colorectal cancer (CRC) than men, however, they have a higher prevalence of right-sided colon cancer (RCC). This is of concern as patients with RCC have the poorest clinical outcomes amongst CRC patients. Aberrant metabolism is a feature of CRC, but it is not known if sex-differences exist. Metabolomics of patient colon tissues (n=236) revealed widespread sex-differences in metabolism depending on tumor location and stage. Tumors from women with RCC were nutrient-deplete, showing enhanced energy production towards asparagine synthesis, and an increase in amino acid uptake. Gene expression data revealed an association between high asparagine synthase expression and poorer overall survival in women with CRC. This is the first study to show sex-differences in tumor tissue metabolism for CRC patients revealing a nutrient-deplete subtype in women with RCC. The results therefore have implications for tumor progression and therapeutic response in women with RCC.</p><p><br></p><p>Linked Studies:</p><p>UPLC-MS HILIC POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1122" target="_blank">MTBLS1122</a></p><p>UPLC-MS HILIC NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1124" target="_blank">MTBLS1124</a></p><p>UPLC-MS RP POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1129" target="_blank">MTBLS1129</a></p><p>UPLC-MS RP NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1130" target="_blank">MTBLS1130</a></p>
2020-08-01 | MSV000085877 | GNPS
Project description:<p>Women have a lower incidence of colorectal cancer (CRC) than men, however, they have a higher prevalence of right-sided colon cancer (RCC). This is of concern as patients with RCC have the poorest clinical outcomes amongst CRC patients. Aberrant metabolism is a feature of CRC, but it is not known if sex-differences exist. Metabolomics of patient colon tissues (n=236) revealed widespread sex-differences in metabolism depending on tumor location and stage. Tumors from women with RCC were nutrient-deplete, showing enhanced energy production towards asparagine synthesis, and an increase in amino acid uptake. Gene expression data revealed an association between high asparagine synthase expression and poorer overall survival in women with CRC. This is the first study to show sex-differences in tumor tissue metabolism for CRC patients revealing a nutrient-deplete subtype in women with RCC. The results therefore have implications for tumor progression and therapeutic response in women with RCC.</p><p><br></p><p>Linked Studies:</p><p>UPLC-MS HILIC POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1122" target="_blank">MTBLS1122</a></p><p>UPLC-MS HILIC NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1124" target="_blank">MTBLS1124</a></p><p>UPLC-MS RP POS assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1129" target="_blank">MTBLS1129</a></p><p>UPLC-MS RP NEG assay is reported in <a href="https://www.ebi.ac.uk/metabolights/MTBLS1130" target="_blank">MTBLS1130</a></p>
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