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Elucidation of the epitranscriptomic RNA modification landscape of chikungunya virus

ABSTRACT:

The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analyzed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyze the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA

modifications in viral RNA genomes.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

SUBMITTER: Belinda Baquero

PROVIDER: MTBLS8697 | MetaboLights | 2024-06-11

REPOSITORIES: MetaboLights

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			Action	DRS
	MTBLS8697	Other
	FILES	Other
	a_MTBLS8697_LC-MS_positive_reverse-phase_metabolite_profiling.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS8697_LC-MS_positive_reverse-phase_metabolite_profiling_v2_maf.tsv	Tabular

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