Dataset Information

Elucidation of the epitranscriptomic RNA modification landscape of chikungunya virus

ABSTRACT:

The genomes of positive-sense (+) single-stranded RNA (ssRNA) viruses are believed to be subjected to a wide range of RNA modifications. In this study, we focused on the chikungunya virus (CHIKV) as a model (+) ssRNA virus to study the landscape of viral RNA modification in infected human cells. Among the 32 distinct RNA modifications analyzed by mass spectrometry, inosine was found enriched in the genomic CHIKV RNA. However, orthogonal validation by Illumina RNA-seq analyses did not identify any inosine modification along the CHIKV RNA genome. Moreover, CHIKV infection did not alter the expression of ADAR1 isoforms, the enzymes that catalyze the adenosine to inosine conversion. Together, this study highlights the importance of a multidisciplinary approach to assess the presence of RNA

modifications in viral RNA genomes.

INSTRUMENT(S): Liquid Chromatography MS - positive - reverse phase

PROVIDER: MTBLS8697 | MetaboLights | 2024-06-11

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	2019LZ023_REME_001_CHK_H4a_250ng.raw	Raw
	2020MR001_MOLU_001_01_CHK_H5.raw	Raw
	2020MR001_MOLU_002_01_CHK_H6.raw	Raw
	2020MR001_MOLU_003_01_NonInf_H1a.raw	Raw
	2020MR001_MOLU_004_01_NonInf_H2.raw	Raw

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Publications

Elucidation of the Epitranscriptomic RNA Modification Landscape of Chikungunya Virus.

Baquero-Pérez Belinda B Bortoletto Enrico E Rosani Umberto U Delgado-Tejedor Anna A Medina Rebeca R Novoa Eva Maria EM Venier Paola P Díez Juana J

Viruses 20240612 6

PMID: 38932237

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