Dataset Information

Identifying host factors associated with DNA replicated during virus infection

ABSTRACT: Viral DNA genomes replicating in cells encounter a myriad of host factors that facilitate or hinder viral replication. Viral proteins expressed early during infection modulate host factors interacting with viral genomes, recruiting proteins to promote viral replication, and limiting access to antiviral repressors. Although some host factors manipulated by viruses have been identified, we know little about pathways exploited during infection and how these differ between viruses. To identify cellular processes manipulated during viral replication, we defined proteomes associated with viral genomes during infection with adenovirus, herpes simplex virus and vaccinia virus. We compared enrichment of host factors between virus proteomes and confirmed association with viral genomes and replication compartments. Using adenovirus as an illustrative example, we uncovered host factors deactivated by early viral proteins, and identified a subgroup of nucleolar proteins that aid virus replication. Our datasets provide valuable resources of virus-host interactions that affect proteins on viral genomes.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Vaccinia Virus Wr Homo Sapiens (human) Human Adenovirus C Serotype 5 (hadv-5) (human Adenovirus 5) Herpesvirales

TISSUE(S): Epithelial Cell, Fibroblast, Osteoid, Cervical Gland

DISEASE(S): Viral Infectious Disease

SUBMITTER: Katarzyna Kulej

LAB HEAD: Matthew D. Weitzman

PROVIDER: PXD007741 | Pride | 2017-10-04

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Homo_Sapiens_UniProt.fasta	Fasta
	Human_adenovirus_C_serotype_5_Swiss_Prot.fasta	Fasta
	Human_herpesvirus1_strain_17.fasta	Fasta
	MaxQuant_1.5.2.8.rar	Other
	Vaccinia_virus_strain_WR10254.fasta	Fasta

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Publications

Identifying Host Factors Associated with DNA Replicated During Virus Infection.

Reyes Emigdio D ED Kulej Katarzyna K Pancholi Neha J NJ Akhtar Lisa N LN Avgousti Daphne C DC Kim Eui Tae ET Bricker Daniel K DK Spruce Lynn A LA Koniski Sarah A SA Seeholzer Steven H SH Isaacs Stuart N SN Garcia Benjamin A BA Weitzman Matthew D MD

Molecular & cellular proteomics : MCP 20171002 12

Viral DNA genomes replicating in cells encounter a myriad of host factors that facilitate or hinder viral replication. Viral proteins expressed early during infection modulate host factors interacting with viral genomes, recruiting proteins to promote viral replication, and limiting access to antiviral repressors. Although some host factors manipulated by viruses have been identified, we have limited knowledge of pathways exploited during infection and how these differ between viruses. To identi ...[more]

PMID: 28972080

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