Dataset Information

A Multi-Omic View of Host-Pathogen-Commensal Interplay in Salmonella-Mediated Intestinal Infection

ABSTRACT: The potential for commensal microorganisms indigenous to a host (the microbiome or microbiota) to alter infection outcome by influencing host-pathogen interplay is largely unknown. We used a multi-omics systems approach, incorporating proteomics, metabolomics, glycomics, and metagenomics, to explore the molecular interplay between the murine host, the pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), and commensal gut microorganisms during intestinal infection with S. Typhimurium. We find proteomic evidence that S. Typhimurium thrives within the infected 129/SvJ mouse gut without antibiotic pre-treatment, inducing inflammation and disrupting the intestinal microbiome (e.g., suppressing Bacteroidetes and Firmicutes while promoting growth of Salmonella and Enterococcus). Alteration of the host microbiome population structure was highly correlated with gut environmental changes, including the accumulation of metabolites normally consumed by commensal microbiota. Finally, the less characterized phase of S. Typhimurium's lifecycle was investigated, and both proteomic and glycomic evidence suggests S. Typhimurium may take advantage of increased fucose moieties to metabolize fucose while growing in the gut. The application of multiple omics measurements to Salmonella-induced intestinal inflammation provides insights into complex molecular strategies employed during pathogenesis between host, pathogen, and the microbiome.

INSTRUMENT(S): 5975C Series GC/MSD (Agilent)

SUBMITTER: Tom Metz

PROVIDER: MTBLS96 | MetaboLights | 2014-06-24

REPOSITORIES: MetaboLights

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Dataset's files

Source:

			Action	DRS
	MTBLS96	Other
	FILES	Other
	a_MTBLS96_salmonella__metabolite_profiling_mass_spectrometry.txt	Txt
	i_Investigation.txt	Txt
	m_MTBLS96_salmonella__metabolite_profiling_mass_spectrometry_v2_maf.tsv	Tabular

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Publications

A Multi-Omic View of Host-Pathogen-Commensal Interplay in Salmonella-Mediated Intestinal Infection.

Deatherage Kaiser Brooke L BL Li Jie J Sanford James A JA Kim Young-Mo YM Kronewitter Scott R SR Jones Marcus B MB Peterson Christine T CT Peterson Scott N SN Frank Bryan C BC Purvine Samuel O SO Brown Joseph N JN Metz Thomas O TO Smith Richard D RD Heffron Fred F Adkins Joshua N JN

PloS one 20130626 6

The potential for commensal microorganisms indigenous to a host (the 'microbiome' or 'microbiota') to alter infection outcome by influencing host-pathogen interplay is largely unknown. We used a multi-omics "systems" approach, incorporating proteomics, metabolomics, glycomics, and metagenomics, to explore the molecular interplay between the murine host, the pathogen Salmonella enterica serovar Typhimurium (S. Typhimurium), and commensal gut microorganisms during intestinal infection with S. Typh ...[more]

PMID: 23840608

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