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Determine purity and quality of IROA labelled glucose

ABSTRACT: We demonstrate the global metabolic analysis ofCaenorhabditis elegansstress responses using a mass-spectrometry-based technique called isotopic ratio outlier analysis (IROA). In an IROA protocol, control and experimental samples are isotopically labeled with 95 and 5%13C, and the two sample populations are mixed together for uniform extraction, sample preparation, and LC-MS analysis.To illustrate the utility of IROA for global metabolomics, we exposed wild-type (N2) worms to a heat shock (30 min heat shock at 33 C), which causes significant, widespread changes in metabolism. We collected and analyzed material from the exometabolome (all material that worms release in the supernatant) and the endometabolome (homogenized total extracts from the worm bodies).

ORGANISM(S): Caenorhabditis Elegans

TISSUE(S): Worms

SUBMITTER: Gregory Stupp

PROVIDER: ST000019 | MetabolomicsWorkbench | Tue Dec 10 00:00:00 GMT 2013

REPOSITORIES: MetabolomicsWorkbench

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