IDH1 and Glioma knockdown idh1 (part II)
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ABSTRACT: Tumor neurospheres were grown in culture until 90% confluent. To collect cells, cells were filtered through 0.45um nylon filters (Fischer Sci 099029) that were prewashed with methanol and ultrapure water using a microanalytical glass filter holder (Fisher Sci 09753E). The cells were filtered through filtration apparatus, then washed with 1mL of distilled water very quickly. Filter paper is taken immediatedly with tweezers and plunged cell side down into 6-well plate filled with liquid nitrogen placed on dry ice. Plate is wrapped in aluminum foil and stored at -80C before the liquid nitrogen evaporates. Samples are shipped on dry ice
ORGANISM(S): Human Homo Sapiens
TISSUE(S): Cells
SUBMITTER: Maureen Kachman
PROVIDER: ST000199 | MetabolomicsWorkbench | Wed Jun 10 00:00:00 BST 2015
REPOSITORIES: MetabolomicsWorkbench
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