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NIH WCMC Pilot & Feasibility Project: Metabolite changes associated with weight loss

ABSTRACT: Targeted metabolomic analyses of oxylipins, endocannabinoids, and ceramides were performed on 18 mouse liver, adipose, hypothalamus, plasma, and muscle samples collected from mice euthanized at 18 weeks following consumption of three diet regimens that induced lean, obese, and weight loss phenotypes. Samples were analyzed by UPLC-MS/MS using a Waters Acquity UPLC and detected on an API 4000 QTrap (AB Sciex, Framingham, MA, USA) by multiple reaction monitoring (MRM) after negative mode electrospray ionization.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Brain, Liver, Muscle, Adipose Tissue, Blood

SUBMITTER: John Newman

PROVIDER: ST000257 | MetabolomicsWorkbench | Fri Jan 16 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

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