Metabolomics

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Primary T Cell Noxa Knockdown (Donor 8) III


ABSTRACT: Labeling of cells was carried out in triplicate with each sample containing 35e6. Cells were deprived of glucose or glutamine for 1 h then resuspended in 10mM [13C1,2] D-glucose labeled or 4 mM [13C5]-U-glutamine labeled medium, respectively. At this time cells were also activated with 5 ug/ml anti-CD3 and anti-CD28. Cells were labeled (and activated) for 16 h when samples were spun down, and cells were resuspended in 200 ul ice cold methanol. For this part of the experiment, the spent medium was collected for analysis. All samples were immediately stored in -80oC.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): T-cells

SUBMITTER: Maureen Kachman  

PROVIDER: ST000370 | MetabolomicsWorkbench | Fri Mar 27 00:00:00 GMT 2015

REPOSITORIES: MetabolomicsWorkbench

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