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Heterologous expression and detection of Apratoxins in E. coli


ABSTRACT: The apratoxin gene cluster was recovered from fosmid DNA library. The gene set responsible for the biosynthesis of the polyketide backbone was introduced in E. coli BAP strain and expressed at 30C for 24 hours. Two sets of control and experimental samples with culture broth pH adjusted at 8 and 12 respectively was extracted with ethyl acetate and dried.

INSTRUMENT(S): Orbitrap

ORGANISM(S): Escherichia Coli E. Coli

TISSUE(S): Bacterial Cells

SUBMITTER: Dimitris Kallifidas  

PROVIDER: ST000446 | MetabolomicsWorkbench | Thu Jul 28 00:00:00 BST 2016

REPOSITORIES: MetabolomicsWorkbench

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