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Fasting wildtype, tfeb -/- knockout, and lmna -/- knockout metabolite profiling of adult zebrafish

ABSTRACT: Inhibition of mechanistic target of rapamycin (mTOR) activity exerts cardioprotective functions. We propose to assess the metabolite profile in zebrafish cardiomyopathy models to test the cardioprotective role of mTOR-TFEB-autophagy and mTOR-lmna- autophagy signaling in heart, liver, muscle, brain, and kidney tissue. In addition mTOR signaling among zebrafish 2 hour post feeding, 24 hour post feeding, and 48 hour post feeding will be profiled. These studes will be used as a baseline and for protocol development before we assess changes in DOX-induced cardiomyopathy.

ORGANISM(S): Danio Rerio Zebrafish

TISSUE(S): Heart, Brain, Liver, Muscle, Kidney

DISEASE(S): Heart Disease

SUBMITTER: Xiaolei Xu

PROVIDER: ST000584 | MetabolomicsWorkbench | Fri Mar 10 00:00:00 GMT 2017

REPOSITORIES: MetabolomicsWorkbench

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