Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Metabolomics of Exhaled Breath Condensate in Decompensated Heart Failure

ABSTRACT: The aim of the study was to test weather characteristic differences or changes in metabolic profile exist between exhaled breath condensate (EBC) and saliva of healthy individuals and heart failure patients. EBC NMR profiling was performed.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Breath

DISEASE(S): Heart Disease

SUBMITTER: Bruce Johnson

PROVIDER: ST000587 | MetabolomicsWorkbench | Fri Apr 07 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	mwtab	Other

Items per page:

1 - 1 of 1

Similar Datasets

Metabolomics of Saliva in Decompensated Heart Failure

Project description:The aim of the study was to test weather characteristic differences or changes in metabolic profile exist between exhaled breath condensate (EBC) and saliva of healthy individuals and heart failure patients. Salival NMR profiling was performed.

2017-04-07 | ST000588 | MetabolomicsWorkbench

miRNA expression in EBC

Project description:Cystic Fibrosis (CF) is the most common life limiting genetic disorder, characterized by chronic respiratory failure secondary to inflammation and chronic bacterial lung infection. Pseudomonas aeruginosa lung infection is associated with more severe lung disease and rapid progression of respiratory failure when compared to Staphylococcus aureus infection. We hypothesized that a specific signature of epigenetic factors targeting specific gene transcripts contributes to the increased morbidity seen in CF patients with chronic Pseudomonas infection. We collected exhaled breath condensate (EBC) from 27 subjects and evaluated miRNA signatures in these samples using commercial PCR array. We identified predicted mRNA targets and associated signaling pathways using Ingenuity Pathway Analysis. We found 11 differentially expressed miRNAs in EBC of patients infected with Pseudomonas aeruginosa compared to EBC from CF patients who were not chronically infected with Pseudomonas aeruginosa.

2019-04-22 | GSE130159 | GEO

Proteomic characterization of human exhaled breath condensate

Project description:The establishment and public sharing of comprehensive proteome maps of human tissues and biofluids are essential to improve biomedical knowledge and to support biomarker discovery studies. In this study, we performed an in-depth proteomics characterization of the exhaled breath condensate (EBC), a non-invasive sample obtained from the condensation of the airway lining fluid. Two pooled samples of EBC, each combined from 10 healthy donors, were lyophilized and their protein content analyzed by high-resolution tandem mass spectrometry-based proteomics. Two “technical” negative control samples were processed in parallel with the pooled samples to correct for exogenous protein contamination and avoid erroneous identifications.

2017-12-06 | PXD007591 | Pride

Assessing the functionality of a novel exhaled breath condensate collector through the molecular analysis of bacteria

Project description:Novel exhaled breath condensate device tests 4 times. Each test generated a single saliva sample and a corresponding exhaled breath condensate sample

| PRJEB7003 | ENA

Exhaled breath condensate biomarkers for the early diagnosis of lung cancer using proteomics

Project description:The present study explored whether the proteomic analysis of exhaled breath condensate (EBC) may provide potential biomarkers for their use in non-invasive screening strategies for the early detection of lung cancer (LC). EBC was collected from 192 individuals (49 control volunteers, 49 risk factor-smoking volunteers, 46 chronic obstructive pulmonary disease patients and 48 LC patients). Using LC-MS/MS a total of 348 different proteins with a different pattern among the four groups were identified in EBC samples. Despite the great variability observed among individuals, significantly more proteins were identified in the EBC from LC patients compared to other groups. Furthermore, the average number of proteins identified per sample was significantly higher in LC patients and Receiver Operating Characteristic curve analysis showed an area under the curve of 0.8, indicating diagnostic value. Proteins frequently detected in EBC, such as dermcidin and hornerin, along with others much less frequently detected, such as hemoglobin and histone isoforms, were identified. Cytokeratins (KRTs) were the most abundant proteins in EBC samples and levels of KRT6A, KRT6B and KRT6C isoforms were significantly higher in samples from LC patients. Moreover, the amount of most KRTs in EBC samples from LC patients showed a significant positive correlation with tumor size. Finally, we used a random forest algorithm to generate a robust model using EBC protein data for the diagnosis of patients with LC. Thus, this study demonstrates that the proteomic analysis of EBC samples is an appropriated approach to develop biomarkers for the diagnosis of lung cancer.

2019-08-16 | PXD006436 | Pride

Proteomics of Exhaled Breath Condensate in COPD patients

Project description:Exhaled breath condensate(EBC) is now a safe and clinically significant measurement which has a huge potential to measure biomarkers in COPD. Previous studies profiled the pooled EBC samples from COPD or control participants due to technological limitations. In our study, 32 COPD patients and 28 control individuals were enrolled, and their EBC were collected. After matching with sex, age and smoking history, EBC samples of 19 COPD patients and 19 control individuals were analyzed using tandem mass tags (TMTs) quantitative mass spectrometry individually.

2019-08-15 | PXD013068 |

Proteome profiling of the exhaled breath condensate after long-term spaceflights

Project description:Abstract: The aim of the study was to analyze exhaled breath condensate (EBC) proteome changes due to the effects of spaceflight factors. All the protocols were approved at the Institute of Biomedical Problems – Russian Federation State Scientific Research Center in the frame of «Protocon» experiment. EBC samples were collected by commercial devices RTube one month before flight (background) at the Yu. A. Gagarin Research and Test Cosmonaut Training Center, immediately after landing of the landing modules in the field (R0), and on the seventh day after landing as a part of medical examination (R+7). Semi-quantitative label-free proteomic analysis of 13 EBC samples collected from 5 Russian cosmonauts before and after long-term (169–199 days) spaceflights were performed and resulted in 164 different proteins. The highest number of proteins was detected in EBC after landing (R0). Pathways enrichment analysis via GO database large group of proteins that take part in keratinization processes (CASP14, DSG1, DSP, JUP, and etc). Nine proteins were KRT2, KRT9, KRT1, KRT10, KRT14, DCD, KRT6C, KRT6A, KRT5 were detected in all groups (background, R0, R+1). A two-sample Welch’s t-test identified the significant changing of KRT2 and KRT9 levels after landing. Enrichment analysis via KEGG database revealed significant participation (2,07E-06) of detected proteins in pathogenic E. coli infection (ACTG1, TUBA1C, TUBA4A, TUBB, TUBB8, YWHAZ). Presumably, presents of this proteins can associated with changing of cosmonauts’ microbial composition. Thus EBC can be used for noninvasive monitoring of health status and respiratory tract pathologies during the spaceflight. The obtained data are important for the development of medicine in extreme situations.

2019-09-27 | PXD014191 | Pride

UHPLC-MS/MS-Based Identity Confirmation of Amino Acids Involved in Response to and Side Effects from Antiseizure Medications

Project description:<p>Real-time breath analysis using secondary electrospray ionization coupled with high-resolution mass spectrometry is a fast and noninvasive method to access the metabolic state of a person. However, it lacks the ability to unequivocally assign mass spectral features to compounds due to the absence of chromatographic separation. This can be overcomed by using exhaled breath condensate and conventional liquid chromatography-mass spectrometry (LC-MS) systems. In this study, to the best of our knowledge, we confirm for the first time the presence of six amino acids (GABA, Oxo-Pro, Asp, Gln, Glu, and Tyr) previously reported to be involved in response to and side effects from antiseizure medications in exhaled breath condensate and by extension in exhaled human breath.</p>

2023-01-26 | MTBLS6760 | MetaboLights

miRNA expression in EBCs

Project description:We analyzed expression of miRNAs in Exhaled Breath Condensates from pediatric patients with sickle cell disease, asthma, sickle cell disease and asthma, and controls

2022-06-11 | GSE205876 | GEO

Expression analysis of inflammatory cytokines in exhaled breath condensates from pediatric patients with sickle cell disease, asthma, sickle cell disease and asthma, and controls

Project description:To analyze expression of inflammatory cytokines in Exhaled Breath Condensates from pediatric patients with sickle cell disease, asthma, sickle cell disease and asthma, and controls

2020-05-08 | GSE150056 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data