Dataset Information

Human Aqueous Humor Phospholipids in Control and Glaucomatous eyes

ABSTRACT: An analysis of phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine and phosphatidylinositol) classes in human control and glaucomatous aqueous humor (AH). Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford’s method, and select samples were confirmed with Densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ Quantum Access Max triple quadrupole mass spectrometer utilizing precursor ion scan (PIS) or neutral ion loss scan (NLS) using appropriate class specific lipid standards in a two-step quantification process. Mass spectrometer data were analyzed using MzMine 2.23.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Eye Tissue

DISEASE(S): Eye Disease

SUBMITTER: Sanjoy Bhattacharya

PROVIDER: ST000616 | MetabolomicsWorkbench | Wed May 31 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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Human Aqueous Humor Phospholipids in Control and Glaucomatous eyes

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