Metabolomics

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Human trabecular meshowrk phospholipid profiles of control and glaucomatous eyes


ABSTRACT: We compared phospholipid (phosphatidylcholine, phosphatidylserine, phosphatidylethanolamine, and phosphatidylinositol) profiles of control and glaucomatous trabecular meshwork (TM) derived from human donors.Lipid extraction was performed using a modification of the Bligh and Dyer method, protein concentrations were determined using the Bradford method, and for select samples confirmed with densitometry of PHAST gels. Lipids were identified and subjected to ratiometric quantification using a TSQ quantum Access Max triple quadrupole mass spectrometer with precursor ion scan (PIS) or neutral ion loss scan (NLS), using appropriate class specific lipid standards.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Eye Tissue

DISEASE(S): Eye Disease

SUBMITTER: Sanjoy Bhattacharya  

PROVIDER: ST000624 | MetabolomicsWorkbench | Thu Jun 01 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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