Project description:Fecal Exosomes from RCD and HFD (isolated from 12 months of their respective diets and purified by sucrose gradient) were orally gavaged to B6 mice for 14 days while they fed HFD. These exosomes were characterized for CD63+A33 duol positivity. After 14 days, liver tissue were harvested. RNA was isolated and sent to invitorgen for Affymetrix array. The purpose of the experiment was to see which set of genes go up or down after these exosomes treatment and their link to metabolic syndrome.
Project description:The purpose of this study was to characterize the gene expression profile of MDA-MB-231 breast cancer cells treated with various SCFA-hexosamine analogs to better understand the role of various modifications to this scaffold. Keywords: SCFA-hexosamine analog comparison
Project description:Consumption of diets rich in fibers has been associated with several beneficial effects on gastrointestinal health. However, detailed studies on the molecular effects of fibers in colon are limited. In this study we investigated and compared the influence of five different fibers on the mucosal transcriptome, and luminal microbiota and SCFA concentrations in murine colon. Mice were fed diets enriched with fibers that differed in carbohydrate composition, namely inulin (IN), oligofructose (FOS), arabinoxylan (AX), guar gum (GG), resistant starch (RS) or a control diet (corn starch) for 10 days. Gene expression profiling revealed the regulation of specific, but also overlapping sets of epithelial genes by each fiber, which on a functional level were mainly linked to cell cycle and various metabolic pathways including fatty acid oxidation, tricarboxylic acid cycle, and electron transport chain. In addition, the transcription factor PPAR was predicted to be a prominent upstream regulator of these processes. Microbiota profiles were distinct per dietary fiber, but the fibers IN, FOS, AX and GG induced a common change in microbial groups. All dietary fibers, except resistant starch, increased SCFA concentrations but to a different extent. Multivariate data integration revealed strong correlations between the expression of genes involved in energy metabolism and the relative abundance of bacteria belonging to the group of Clostridium cluster XIVa, that are known butyrate producers. These findings illustrate the potential of multivariate data analysis to unravel simple relationships in complex systems. Keywords: Expression profiling by array Mice received a control diet, or a diet supplemented with 10% dietary fibers for 10 days. After an overnight fast colon was removed, epithelial cells were scraped off, and subjected to gene expression profiling.
Project description:We aim to establish NAFLD model of Zebrafish. Zebrafish larvae fed with high cholesterol diet,high fructose diet and overfeed diet to induce liver steatosis. RNA-seq was employed to analyze the effects of different diets on NAFLD development.