Metabolomics

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Dynamics of metabolism, proliferation and differentiation in Toxoplasma gondii mutants deficient in glycolysis and gluconeogenesis


ABSTRACT: The focus of this study was to profile the metabolic fates of glucose and glutamine in wild type, glycolytic mutant (hexokinase KO) and gluconeogenesis mutant (PEPCK1 KO) tachyzoite stage T. gondii parasites. 13C6-glucose and 13C5-15N1-glutamine were used as metabolic tracers. Metabolites from glycolysis, pentosephosphate pathway and Kerbs cycle were identified and their isotopomer abundance was quantified in order to visualize metabolic flux across the indicated pathways. A time course (from 0 minutes to 120 minutes) analysis was carried out in the labeling experiments. Here, host cell free extracellular tachyzoite stage T. gondii parasites were used.

ORGANISM(S): Toxoplasma Gondii

TISSUE(S): Cells

DISEASE(S): Parasitic Infection

SUBMITTER: Dhanasekaran Shanmugam  

PROVIDER: ST000817 | MetabolomicsWorkbench | Sat Jul 22 00:00:00 BST 2017

REPOSITORIES: MetabolomicsWorkbench

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