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Retinal ganglion cells lipid profiling

ABSTRACT: The aim of this project was to characterize lipid profiles of the retinal ganglion cells (RGCs) with different regenerative capacity. RGCs were FACS-sorted (7,000 cells/sample) from mice of OPN4 Cre tdT and Thy1-CFP genotype. The treatment conditions included intact, optic nerve crush (ONC) and ONC plus CNTF to promote regeneration. Samples were then subject to lipid extraction and analyzed using LC-MS/MS, followed by identification and relative quantification in LipidSearch software.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Eye Tissue

DISEASE(S): Eye Disease

SUBMITTER: Sanjoy Bhattacharya

PROVIDER: ST001105 | MetabolomicsWorkbench | Mon Nov 26 00:00:00 GMT 2018

REPOSITORIES: MetabolomicsWorkbench

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