Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Development and Characterisation of a Novel Class of Aroyl Guanidine Containing Anti-Trypanosomal Compounds

ABSTRACT: The mode of action of a novel class of aroyl guanidine containing anti-Trypanosomal compounds was investigated using an unbiased metabolomics approach. Trypanosoma brucei parasites were incubated for five hours with the test compounds at 1 µM concentration. Analysis of cell pellets allowed reproducible detection of diverse metabolites from a range of metabolic pathways.

ORGANISM(S): Trypanosoma Brucei Brucei Trypanosoma Brucei

TISSUE(S): Cultured Cells

DISEASE(S): Sleeping Sickness

SUBMITTER: Darren Creek

PROVIDER: ST001276 | MetabolomicsWorkbench | Sat Nov 16 00:00:00 GMT 2019

REPOSITORIES: MetabolomicsWorkbench

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
	mwtab	Other

Items per page:

1 - 1 of 1

Similar Datasets

Metabolomics-based profiling of the mode of action of Pathogen Box compounds in Trypanosoma brucei (part-I)

Project description:The mode of action of anti-Trypanosomal compounds from the Pathogen Box was investigated using an unbiased metabolomics approach. Trypanosoma brucei parasites were incubated for five hours with the test compounds at 0.5 µM concentration. Analysis of cell pellets allowed reproducible detection of diverse metabolites from a range of metabolic pathways.

2019-11-16 | ST001274 | MetabolomicsWorkbench

Metabolomics-based profiling of the mode of action of Pathogen Box compounds in Trypanosoma brucei (part-II)

2019-11-16 | ST001275 | MetabolomicsWorkbench

Novel anti-virulence compounds disrupt exotoxin expression in MRSA

Project description:Hemolysins are lytic exotoxins expressed in most strains of S. aureus, but hemolytic activity varies between strains. We have previously reported several novel anti-virulence compounds that disrupt the S. aureus transcriptome, including hemolysin gene expression. This report delves further into our two lead compounds, loratadine and a structurally related brominated carbazole, and their effects on hemolysin production in methicillin-resistant S. aureus (MRSA). To gain understanding into how these compounds affect hemolysis, we analyzed these exotoxins at the DNA, RNA, and protein level after in vitro treatment. While lysis of red blood cells varied between strains, DNA sequence variation did not account for it. We hypothesized that our compounds would modulate gene expression of multiple hemolysins in two hospital-acquired strains of MRSA, both with staphylococcal cassette chromosome mec (SCCmec) type II. RNA-seq analysis of differential gene expression in untreated and compound-treated cultures revealed hundreds of differentially expressed genes, with a significant enrichment in genes involved in hemolysis. The brominated carbazole and loratadine both displayed the ability to reduce hemolysis in strain 43300 but displayed differential activity in strain USA100. These results corroborate gene expression studies as well as western blots of alpha hemolysin. Together, this work suggests that small molecules may alter exotoxin production in MRSA but that the directionality and/or magnitude of the difference are likely strain dependent.

2024-05-15 | GSE267020 | GEO

Novel anti-virulence compounds disrupt exotoxin expression in MRSA

Project description:Novel anti-virulence compounds disrupt exotoxin expression in MRSA

| PRJNA1109356 | ENA

Anti-lymphoma activities of different compounds

Project description:Anti-lymphoma activities of different compounds

| PRJNA639539 | ENA

Anti-inflammatory compounds in SCI

Project description:A screen of 5 anti-inflammatory compounds for their effects in explanted, cultured rat spinal cord slices. All injured (explanted) cords are cultured for 4 hrs. Keywords: ordered

2003-10-03 | GSE633 | GEO

Anti-inflammatory compounds in SCI

Project description:A screen of 5 anti-inflammatory compounds for their effects in explanted, cultured rat spinal cord slices. All injured (explanted) cords are cultured for 4 hrs.

2010-06-05 | E-GEOD-633 | biostudies-arrayexpress

Determination of mode of action of anti-malalrial drugs using untargeted metabolomics

Project description:The mode of action of a representative active compound was investigated using an unbiased metabolomics approach, which has previously been shown to reveal both novel and established modes of action of antimalarials (Creek et al 2016, DOI: 10.1128/AAC.01226-16). The active antimalarial OSM-S-313, and the inactive analogue OSM-S-291, were incubated with trophozoite stage P. falciparum parasites for five hours alongside reference compounds including atovaquone (ATV), chloroquine (CQ), dihydroartemisisin (DHA) and three PfATP4 inhibitors, MMV00073, MMV397264 and MMV390482. Metabolomics analysis of cell pellets and spent media allowed reproducible detection of diverse metabolites from a range of metabolic pathways, with the most significant OSM-S-313-induced perturbations observed within peptide, lipid and energy metabolism, suggesting a specific impact on parasite metabolism.

2018-08-13 | ST001033 | MetabolomicsWorkbench

MS-CETSA Target Identification of MMV compounds: MMV007127, MMV665886

Project description:ITDR MS-CETSA was conducted on MMV compounds-treated live parasites and lysate of P. falciparum trophozoites in order to identify potential drug protein targets. MMV compounds: MMV007127, MMV665886.

2024-01-23 | PXD048768 | Pride

Profiling of novel p53-inducing compounds

Project description:We describe six novel lead compounds activating the p53 tumor suppressor pathway. Transcriptional profiling indicated that they invoked highly similar responses involving p53 and cell cycle pathways and DNA damage response signaling. Keywords: Stress response Six compounds and one vehicle control, with four biological replicates each, for a total of 28 samples.

2010-10-01 | E-GEOD-12666 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data