Diel Metabolites in the North Pacific Subtropical Gyre (KM1513)

ABSTRACT: Diverse organisms within the marine microbial communities show 24-hour cycles of gene expression, likely driven by the need to harness energy from sunlight and to cope with dramatic fluctuations in solar radiation over the course of the day. Metabolites are the direct product of metabolic activity; they are therefore expected to both reflect and influence the daily cycle of the microbial community. Here we measure the intracellular metabolome of the microbial community of the North Pacific Subtropical Gyre, sampled at 4-hour intervals for 8 days. Concentrations of some metabolites common to many organisms exhibit diel periodicity, revealing synchrony of community-level metabolism. Comparing these data to gene expression data reveals temporal offsets between gene transcription and cellular activity, and ties some metabolites to the activities of specific organisms. For example, the dramatic fluctuations of the disaccharide trehalose likely reflect the daily cycles of {Crocosphaera}, a photosynthesizing cyanobacteria that needs to store energy during the day to fuel nighttime nitrogen-fixation. This study illustrates how pairing multiple types of 'omics and environmental data can provide insight into how the activities of individual organisms lead to community functions such as net primary productivity and nitrogen fixation.

ORGANISM(S): Ilyonectria Mors-panacis Ilyonectria Robusta Neonectria Obtusispora Ilyonectria Torresensis Ilyonectria Mors-panacis;ilyonectria Rufa;ilyonectria Robusta;ilyonectria Torresensis;ilyonectria Estremocensis;neonectria Obtusispora Ilyonectria Estremocensis Ilyonectria Rufa

TISSUE(S): Suspended Marine Particulate Matter

SUBMITTER: Angela Boysen  

PROVIDER: ST001356 | MetabolomicsWorkbench | Mon Mar 23 00:00:00 GMT 2020

REPOSITORIES: MetabolomicsWorkbench