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Branched-chain alpha-ketoacids are preferentially reaminated and activate protein synthesis in the rat heart

ABSTRACT: Branched-chain amino acids (BCAA) and their cognate α-ketoacids (BCKA) are elevated in an array of cardiometabolic diseases. Here we demonstrate that the major metabolic fate of uniformly-13C-labeled α-ketoisovalerate ([U-13C]KIV) in the heart is reamination to valine. Activation of cardiac branched-chain α-ketoacid dehydrogenase (BCKDH) by treatment with the BCKDH kinase inhibitor, BT2, does not impede the strong flux of [U-13C]KIV to valine.

ORGANISM(S): Rat Rattus Norvegicus

TISSUE(S): Heart

DISEASE(S): Obesity

SUBMITTER: Jacquelyn Walejko

PROVIDER: ST001665 | MetabolomicsWorkbench | Tue Jan 26 00:00:00 GMT 2021

REPOSITORIES: MetabolomicsWorkbench

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