Metabolomics

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Targeted Sphingolipid analysis of HeLa silenced for or overexpressing GOLPH3 or LCS


ABSTRACT: A group of sequentially-acting enzymes operating at the branchpoint among sphingolipid synthetic pathways binds the Golgi-localised oncoprotein GOLPH3. GOLPH3 sorts these enzymes into vesicles for intra-Golgi retro-transport, acting as a component of the cisternae inter-conversion mechanisms. Through these effects, GOLPH3 controls the sub-Golgi localisation, and the lysosomal degradation rate of specific enzymes. Here we evaluated the impact of overexpressing or silencing GOLPH3 or its client enzyme lactosylceramide synthase (LCS) on the sphingolipid composition of HeLa cells by targeted lipid analysis.

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Hela Cells

SUBMITTER: Giovanni D'Angelo  

PROVIDER: ST001673 | MetabolomicsWorkbench | Fri Jan 29 00:00:00 GMT 2021

REPOSITORIES: MetabolomicsWorkbench

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