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Systemic host inflammation induces stage-specific transcriptomic modification and slower maturation in malaria parasites (part I)

ABSTRACT: Previous reports suggest that the maturation rate of malaria parasites within red blood cells (RBC) is not constant for a given species in vivo. For instance, maturation can be influenced by host nutrient status or circadian rhythm. Here we observed in mice that systemic host inflammation, induced by lipopolysaccharide (LPS) conditioning or ongoing acute malaria infection, slowed the progression of a single cohort of parasites from one generation of RBC to the next. LPS-conditioning and acute infection both triggered substantial changes to the metabolomic composition of plasma in which parasites circulated. This altered plasma directly slowed parasite maturation in a manner that could not be rescued by supplementation, consistent with the presence of inhibitory factors. Single-cell transcriptomic assessment of mixed parasite populations, exposed to a short period of systemic host inflammation in vivo, revealed specific impairment in the transcriptional activity and translational capacity of trophozoites compared to rings or schizonts. Thus, we provide in vivo evidence of transcriptomic and phenotypic plasticity of asexual blood-stage Plasmodium parasites when exposed to systemic host inflammation

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Blood

DISEASE(S): Malaria

SUBMITTER: Thomas Stoll

PROVIDER: ST001899 | MetabolomicsWorkbench | Mon Aug 09 00:00:00 BST 2021

REPOSITORIES: MetabolomicsWorkbench

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