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Systemic impact of the expression of the mitochondrial alternative oxidase on Drosophila development

ABSTRACT: Despite the beneficial effects shown when the mitochondrial alternative oxidase AOX from Ciona intestinalis (Tunicata: Ascidiacea) is xenotopically expressed in mammalian and insect models, important detrimental outcomes have also been reported, raising concerns regarding its envisioned deployment as a therapy enzyme for human mitochondrial and related diseases. We have shown previously that AOX-expressing flies present a dramatic drop in pupal viability when the larvae are cultured on a low nutrient (LN) diet, indicating that AOX interferes with normal developmental metabolism. Here, we applied the metabolomics approach to gain insights into the molecular basis of the fatal developmental interaction between AOX expression and LN diet. We investigated the whole-body metabolome of AOX-expressing and control larvae cultured on SD and LN diets.

ORGANISM(S): Drosophila Melanogaster Fruit Fly

TISSUE(S): Larvae

SUBMITTER: Eric Dufour

PROVIDER: ST002069 | MetabolomicsWorkbench | Sat Jan 29 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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