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Lipidomic characterization of Jurkat-derived T cell line in which the chaperonin complex CCT has been partially silenced

ABSTRACT: When the chaperonin complex CCT is partially silenced in Jurkat-derived T cell line J77 E61, we observe changes in the production of exosomes and in their composition. Thus, to explore the bases of these alterations and find possible new mechanisms of exosome biosynthesis regulation we characterized the lipidome content in cells where the chaperonin complex CCT was partially silent (CCT) and controls (CTRL). We analyzed 5 biological replicates containing 3 × 106 cells using LC-MS in positive and negative polarity mode. For quality control, 5 QCs samples and 4 blanks were also included in the analysis (total 19 samples and 2 groups).

ORGANISM(S): Human Homo Sapiens

TISSUE(S): T-cells

SUBMITTER: Alessia Ferrarini

PROVIDER: ST002185 | MetabolomicsWorkbench | Fri Jun 03 00:00:00 BST 2022

REPOSITORIES: MetabolomicsWorkbench

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