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Gene expression of native Jurkat human leukemia cell line following 8h Brd4 inhibition by MMQO

ABSTRACT: Basal Jurkat cell gene expression and MMQO treated Jurkat cell gene expression

ORGANISM(S): Homo sapiens

PROVIDER: GSE100361 | GEO | 2018/06/22

REPOSITORIES: GEO

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Gene expression of native Jurkat human leukemia cell line following 3h BET inhibition by MMQO or 3h HDAC inhibition by TSA

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Transcription profiling of Jurkat T cell line and mouse thymocytes

Project description:Signal transduction pathways guided by cellular receptors commonly exhibit low-level constitutive signaling in a continuous, ligand-independent manner. The dynamic equilibrium of positive and negative regulators establishes such a tonic signal. Ligandindependent signaling by the precursors of mature antigen receptors regulates development of B- and T-lymphocytes. Here we describe a basal signal controlling gene expression profiles in the Jurkat T cell line and mouse thymocytes. Using DNA microarrays and Northern blots to analyze unstimulated cells, we demonstrate that expression of a cluster of genes, including RAG-1 and RAG2, is repressed by constitutive signals requiring the adapter molecules LAT and SLP-76. This TCR-like pathway results from constitutive low-level activity of Erk and Abl kinases. Inhibition of Abl by the drug STI-571 or events upstream of Erk increases RAG-1 expression. Our data suggest that physiologic gene expression programs depend upon tonic activity of signaling pathways independent of receptor ligation.

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Project description:Vitamin K1 (VK1), an essential nutrient related to coagulation, has been proved to have anticancer properties in various cancer cells. However, little is known about the effects of VK1 on hematologic malignancies. The aim of the study was to evaluate the cytotoxic effects of VK1 on acute T-cell lymphocytic leukemia Jurkat cells (Jurkat T cells). In addition, VK1 induced changes of gene expression in Jurkat T cells were also observed.

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