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Biomolecular condensates create phospholipid-enriched microenvironments (Part 1)

ABSTRACT: Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Liver

SUBMITTER: Jason Dumelie

PROVIDER: ST002349 | MetabolomicsWorkbench | Fri Nov 04 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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Biomolecular condensates create phospholipid-enriched microenvironments (Part 2)

Project description:Proteins and RNA are able to phase separate from the aqueous cellular environment to form sub-cellular compartments called condensates. This process results in a protein-RNA mixture that is chemically distinct from the surrounding aqueous phase. Here we use mass spectrometry to characterize the metabolomes of condensates. To test this, we prepared mixtures of phase-separated proteins and cellular metabolites and identified metabolites enriched in the condensate phase. These proteins included SARS-CoV-2 nucleocapsid, as well as low complexity domains of MED1 and HNRNPA1.

2022-11-15 | ST002352 | MetabolomicsWorkbench

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