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Human primary astrocytes finger- and footprinting metabolomics indicate biochemical alterations under ayahuasca treatment

ABSTRACT: Ayahuasca (Aya) is a psychotropic Amazonian beverage formulated from the combination of the Banisteriopsis caapi vine and the Psychotria viridis leaves in a water decoction. Aya is legally used in Latin American countries and used in Brazil for religious, cultural, and therapeutic purposes. Its properties constitute a bio-psycho-social-spiritual model involving effects from β-carboline-derived alkaloids, present in the vine, and N,N-dimethyltryptamine (DMT), a tryptamine-derived alkaloid present in the leaves, which act together in the central nervous system (CNS). Few technical-scientific studies have been conducted to understand the effects of this brew in the metabolism. Therefore, this work aims to investigate an in vitro primary astrocyte lineage model by untargeted metabolomics evaluations of two cellular subfractions: secretome and intracellular content after Aya treatment, where DMT and other β-carbolines were previously quantified. Metabolomics analysis was performed by UHPLC-MS/MS, followed by MS-Dial data processing and statistical analysis to identify metabolites and biochemical alterations related to Aya treatment. Aya doses were applied to the cell cultures considering DMT concentrations of 1, 10 and 20 µM, which are in agreement with non-toxic and toxic DMT threshold assays in primary human astrocyte cells viability

ORGANISM(S): Human Homo Sapiens

TISSUE(S): Cultured Cells

SUBMITTER: Flavia Zandonadi

PROVIDER: ST002401 | MetabolomicsWorkbench | Fri Nov 11 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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