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Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation:Part 2

ABSTRACT: Preclinical studies of muscle contractile function often employ ex vivo preparations of the soleus and/or extensor digitorum longus (EDL) muscles which are relatively easy to prepare and represent slow and fast fiber properties, respectively. Therefore, the current study sought to examine the utility of this preparation for understanding the metabolic fuel utilization in isolated resting mouse muscles at room temperature. 13C-labeling in both muscle types was performed using three fuels: glucose, pyruvate, and acetate, followed by NMR-based metabolomics analyses. Incubating 13C-labeled substrates in the isolated skeletal muscles makes it possible to examine TCA cycle flux and substrate selection by these muscles.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Muscle

SUBMITTER: Ram Khattri

PROVIDER: ST002426 | MetabolomicsWorkbench | Mon Oct 31 00:00:00 GMT 2022

REPOSITORIES: MetabolomicsWorkbench

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Isolated murine skeletal muscles utilize pyruvate over glucose for oxidation-Part 1

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