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Tryptophan metabolites in mouse serum

ABSTRACT: Analyzing murine sera to investigate into which tryptophan metabolites in the host circulation are derived from diet, host metabolism and/or the gut microbiome. Blood was collected from the portal vein of conventional C567BL/6J mice and sera analyzed by targeted LC-MS. To test this hypothesis and evaluate the necessity of microorganisms to produce these examined tryptophan metabolites, sera were collected from germ-free (GF) mice and subjected to the same targeted metabolomic analysis. Having generated Trp metabolite profiles from sera, we asked if altering the diet would significantly change circulating concentrations. One of two groups of conventional C57BL/6J mice with ad libitum access to a standard laboratory chow were switched onto a semi-purified diet (AIN-93G) while the second remained on standard chow. After one week, portal vein blood was collected and analyzed using targeted LC-MS.

ORGANISM(S): Mouse Mus Musculus

TISSUE(S): Blood

SUBMITTER: FANGCONG DONG

PROVIDER: ST002463 | MetabolomicsWorkbench | Fri Jan 27 00:00:00 GMT 2023

REPOSITORIES: MetabolomicsWorkbench

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