Dataset Information

Metaproteomes of intestinal microbiomes from mice fed different sources of dietary protein

ABSTRACT: Diet plays a major role in altering the composition and function of the gut microbiota. Previously most studies have focused on the effects of fiber, fat, and different amounts of protein on the gut microbiota. In this study we investigated how different sources of protein affect the gut microbiota of mice. We fed conventional and germ-free C57BL/6J mice a series of defined diets where the source of dietary protein was the key difference, which made up twenty or forty percent of the diet. The dietary protein sources used were purified protein. The diets were fed to the same mice for one week each with a fecal sample collected at the end of each week. The diets were fed in this order: standard chow, 20% soy, 20% casein, 20% rice, 40% soy, 20% yeast, 40% casein, 20% pea, 20% egg white protein, 20% chicken bone broth, and lastly at the end of the experiment half of the mice were fed the 20% soy and half the mice the 20% casein diet again as a control. We did not collect fecal samples for the chicken bone broth diet as the diet was stopped prematurely due to diet intolerance. 12 germ-free mice (6 female, 6 male) in four cages were used. 12 mice with a conventional gut microbiota in four cages were used (6 female, 6 male). One germ-free mouse was found dead after diet 5 (20% yeast) and one conventional mouse was sacrificed after the second diet (20% casein). No sample could be collected from one of the conventional mice after the 20% egg white diet.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Bos Taurus (bovine) Cyberlindnera Jadinii Gallus Gallus Gallus Danio Rerio (zebrafish) (brachydanio Rerio) Lathyrus Aphaca Oryza Sativa Subsp. Japonica (rice) Zea Mays (maize) Triticum Aestivum (wheat) Glycine Max Mus Musculus (mouse) Mouse Gut Metagenome

SUBMITTER: J. Alfredo Blakeley Ruiz

LAB HEAD: Manuel Kleiner

PROVIDER: PXD041586 | Pride | 2024-12-18

REPOSITORIES: pride

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Dietary protein source alters gut microbiota composition and function.

Blakeley-Ruiz J Alfredo JA Bartlett Alexandria A McMillan Arthur S AS Awan Ayesha A Vanhoy Walsh Molly M Meyerhoffer Alissa K AK Vintila Simina S Maier Jessie L JL Richie Tanner T Theriot Casey M CM Kleiner Manuel M

bioRxiv : the preprint server for biology 20250112

The source of protein in a persons diet affects their total life expectancy. However, the mechanisms by which dietary protein sources differentially impact human health and life expectancy are poorly understood. Dietary choices have major impacts on the composition and function of the intestinal microbiota that ultimately modulate host health. This raises the possibility that health outcomes based on dietary protein sources might be driven by interactions between dietary protein and the gut micr ...[more]

PMID: 38617292

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	20CHXR1.raw	Raw
	20CHXR2.msf	Msf
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	20CHXR3.msf	Msf