Project description:The objective of the protject is to develop an immune cell-enrich library-assisted DIA method that enables the comprehensive identification and quantification of immunological proteins from micro-scale samples with high sensitivity. In the project, six immune cell subtype-specific libraries including CD8+ and CD4+ T cell, B cell, NK cell, DC and MP were established, and the six subtype libraries can be consolidated into a larger library termed the immune cell-enriched library. By integrating either a single cell-type specific library or multiple libraries into the conventional library-based DIA search, the method enhances the identification coverage of the immune landscape for micro-scale colonic polyps in CRC mice. Application of the method on the MLNs of the CRC mice offers an enlarged TME landscape that reveals proteomic changes in the MLN from different tumor growth of progrssion.
Project description:There have been clinical reports of splenectomy as a treatment option for immune-mediated hematologic diseases in humans and dogs. We focused on the efficacy of splenectomy as a treatment for dogs with precursor-targeted immune-mediated anemia. We collected serum samples of pre and post splenectomy in these dogs to investigate the factors that might improve non-regenerative anemia after splenectomy.
Project description:The impact of CRTAM modulation on the balance between gut microbiota and the mucosal immune system under both homeostatic conditions and during enteric infections