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Chamaecyparis obtusa

ABSTRACT: Transcriptome analysis of the Hinoki differentiating xylem using SOLiD paired-end sequencing

PROVIDER: PRJDB1823 | ENA |

REPOSITORIES: ENA

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			Action	DRS
	DRR013015_1.fastq.gz	Fastqsanger.gz
	DRR013015_2.fastq.gz	Fastqsanger.gz
	DRR013016_1.fastq.gz	Fastqsanger.gz
	DRR013016_2.fastq.gz	Fastqsanger.gz
	DRR013017_1.fastq.gz	Fastqsanger.gz

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Chamaecyparis obtusa

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Chamaecyparis obtusa

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Project description:The complete plastid genome of Chamaecyparis obtusa was sequenced and analyzed in this study. It was found to be 126,821 bp long. The guanine-cytosine content of the whole genome was 35.1%, and there were 83 unique protein-coding genes, 33 tRNAs, and 4 rRNAs. Furthermore, an overlap of 120 bp was found (including trnQ-UUG). The order and organization of these genes were consistent with those of other complete plastid genomes from the Cupressaceae. A phylogenetic tree was constructed according to the 83 protein-coding genes found, which demonstrated that C. obusa had sister relationships within the genus Chamaecyparis.

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Development and characterization of microsatellite primers for Chamaecyparis obtusa (Cupressaceae).

Project description:Although several microsatellite markers of Chamaecyparis obtusa (Cupressaceae) have been reported in previous studies, we newly developed and evaluated polymorphic microsatellite markers of C. obtusa, an economically important species in Korea as a major forestation tree species, for further genetic studies. Microsatellite-enrichment libraries were constructed according to the selective hybridization method. From this output, we obtained 10 polymorphic and five monomorphic microsatellite markers for C. obtusa. Five of these developed markers were successfully amplified for C. pisifera, a related species. These microsatellite markers can be valuable tools for further genetic studies of C. obtusa, and gathered genetic information may be useful for breeding program management.

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Secondary Metabolites with Antimicrobial Activities from Chamaecyparis obtusa var. formosana.

Project description:Seven new compounds, including one dimer novel skeleton, chamaecyformosanin A (1); three diterpenes, chamaecyformosanins B-D (2-4); one sesquiterpene, chamaecyformosanin E (5); and two monoterpenes, chamaecyformosanins F and G (6 and 7) were isolated from the methanol extract of the bark of Chamaecyparis obtusa var. formosana. Their structures were established by the mean of spectroscopic analysis and the comparison of NMR data with those of known analogues. Their structures were elucidated on the basis of physicochemical evidence, in-depth NMR spectroscopic analysis, and high-resolution mass spectrometry. Furthermore, the isolated compounds were subjected to an evaluation of their antimicrobial activity. Metabolites 1, 3, and 4 present antibacterial activities. It is worth mentioning that the chemical composition of the bark of C. obtusa var. formosana has never been studied in the past. This is the first time the barks from C. obtusa var. formosana were studied and two new skeleton compounds, 1 and 7, were obtained.

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Chamaecyparis obtusa var. formosana

Project description:Chamaecyparis obtusa var. formosana Raw sequence reads

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Project description:We focused on RNA-seq-based full transcriptome responses to PtrSND1-B1 overexpression at 7, 12, and 25 h in stem defferentiating xylem (SDX) protoplasts We transfected PtrSND1-B1 and sGFP into stem differentiating xylem protoplasts and performed RNA-seq to reveal the whole transcriptome.

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Effective Extraction of Limonene and Hibaene from Hinoki (Chamaecyparis obtusa) Using Ionic Liquid and Deep Eutectic Solvent.

Project description:The essential oils of hinoki (Chamaecyparis obtusa) leaves have anti-bacterial, anti-fungal, and relaxation properties that are likely associated with the major components such as sabinene, α-terpinyl acetate, limonene, elemol, myrcene, and hibaene. The present study describes the use of a cellulose-dissolving ionic liquid (IL) [C2mim][(MeO)(H)PO2] and low-toxicity solvents called betaine-based deep eutectic solvents (DESs) for the efficient extraction of hinoki essential oils. As a control method, organic solvent extraction was performed using either hexane, ethyl acetate (EtOAc), or acetone at 30 °C for 1 h. Both the experimental and control methods were conducted under the same conditions, which relied on partial dissolution of the leaves using the IL and DESs before partitioning the hinoki oils into the organic solvent for analysis. Quantitative analysis was performed using gas chromatography-mass spectrometry (GC-MS) in selected ion monitoring (SIM) mode. The results indicated that extraction using the [C2mim][(MeO)(H)PO2]/acetone bilayer system improved the yields of limonene and hibaene, 1.5- and 1.9-fold, respectively, when compared with the control method. In addition, extraction using betaine/l-lactic acid (molar ratio 1:1) gave the greatest yields for both limonene and hibaene, 1.3-fold and 1.5-fold greater, respectively, than when using an organic solvent. These results demonstrate the effective extraction of essential oils from plant leaves under conditions milder than those needed for the conventional method. The less toxic and environmentally begin DESs for the extraction are also applicable to the food and cosmetic industries.

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