Mentha spicata
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REPOSITORIES: ENA
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Project description:Peltate glandular trichomes from /Mentha spicata /were purified on a Percoll gradient and soluble and membrane proteins were trypsinized and the peptides separated by nano-LC fractionation and analyzed by MALDI-MS/MS. The vast majority of the 1,666 proteins identified was housekeeping proteins or involved in primary metabolism. However, 57 were predicted to be involved in secondary metabolism. Of these, 21 were involved in the synthesis of phenylpropanoids and phenolics and 32 in terpenoid synthesis. Of the 14 membrane transporters identified, the 11 ATP-binding cassette transporters provide good material for assessing whether active transport is required for the transfer of monoterpenoid intermediates between cellular compartments and for the secretion of the final products into the subcuticular storage cavity. In conclusion, this proteome analysis of /M. spicata/ peltate trichomes has identified several candidate proteins that might be involved in terpenoid synthesis and transport. MALDI-MS/MS analyses were performed as described previously (Szopinska et al., 2011). MS data from the soluble and microsomal fractions were pooled and analyzed as a single data set using ProteinPilotTM software (AB SCIEX, v.4.0.8085). Protein identification was based on ParagonTM algorithm v.4.0 and used the Viridiplantae part of the whole NCBInr protein database (downloaded on April 14th, 2013). This algorithm in ProteinPilot was used with “identification” as the sample type, “MMTS” as cysteine modification, “4800 TOF/TOF” as the instrument, and the "Thorough" preset search setting. All reported proteins were identified with 95% or greater confidence, as determined by ProteinPilot unused scores (greater than 1.3) using the most stringent threshold of false positive discovery rate, i.e. below 10%. Protein grouping performed by ProteinPilotTM removed redundant hits.
2013-10-17 | PXD000352 | Pride
Project description:Mentha spicata Transcriptome or Gene expression
| PRJNA215365 | ENA
Project description:Glandular trichome transcriptome sequencing of genus Mentha.
| PRJNA245825 | ENA
Project description:Trichomes are found on the leaf surface of many plant species. They can be classified into either glandular or non-glandular types. Glandular trichomes are usually multicelluar structures and have long been known to participate in defense against pathogens through the production of specialized chemical products. Non-glandular trichomes, such as those in Arabidopsis thaliana, have been hypothesized to have roles in physical defense against insects, UV protection, detoxification, and heat dispersal. However, it has been difficult to separate a physical role from other possible functions for non-glandular trichomes. We have taken advantage of several mutants in the trichome developmental pathway and gene expression analyses to identify a set of genes expressed predominantly in Arabidopsis trichomes. Keywords: comparing gene expression between wild type and mutants
2008-04-30 | GSE9597 | GEO
Project description:Trichomes are found on the leaf surface of many plant species. They can be classified into either glandular or non-glandular types. Glandular trichomes are usually multicelluar structures and have long been known to participate in defense against pathogens through the production of specialized chemical products. Non-glandular trichomes, such as those in Arabidopsis thaliana, have been hypothesized to have roles in physical defense against insects, UV protection, detoxification, and heat dispersal. However, it has been difficult to separate a physical role from other possible functions for non-glandular trichomes. We have taken advantage of several mutants in the trichome developmental pathway and gene expression analyses to identify a set of genes expressed predominantly in Arabidopsis trichomes. Experiment Overall Design: We compared the gene expression between the wild type and three trichomeless mutants: gl1, ttg1 and gl3egl1 to see which genes' transcripts are enriched in trichomes.
2008-06-16 | E-GEOD-9597 | biostudies-arrayexpress
Project description:Glandular trichomes (GT) are specialized cell factories that have the capacity to produce large amounts of metabolites which can amount to over 10% of the leaf dry weight. The specific expression of secondary metabolite pathways in glandular trichomes has facilitated their elucidation. However, little is known about the connection between central carbon and specialized metabolism in these cells. To address this question, we used the type VI glandular trichomes from a cultivated (Solanum lycopersicum LA4024) and a wild tomato accession (Solanum habrochaites LA1777) as a model. Our study is based on metabolomics, transcriptomics, proteomics and 13C-labeling datasets of trichome and leaves samples. This comparative analysis allowed us to identify specific features of trichomes in comparison to leaves. Tomato type VI trichomes are photosynthetic but acquire their carbon essentially from the leaves in the form of sucrose. Relatively high expression levels of photosystem genes and comparatively low expression of Calvin-cycle and photorespiration genes indicate that photosynthesis is used primarily to produce energy and reducing power to drive the high metabolic activity. High levels of polyunsaturated fatty acids, oxylipins, and of oxidized glutathione and high transcript and protein levels of associated enzymes further indicate a distinct capacity of GT to cope with oxidative stress. Enzymes which are involved in the supply of key precursors for the production of terpenoids both from the cytosol and in the chloroplasts are markedly increased. Finally, some enzymes that are typically associated with C4-photosynthesis are highly expressed in trichomes, suggesting that CO2 recycling processes may increase the efficiency of C usage in these cells. Based on this information we propose a model on how the glandular trichomes achieve high metabolic efficiency.
2017-07-04 | PXD003154 | Pride