Project description:Aster yellows phytoplasma strain Hyd35 (16SrI-B) in micropropagated periwinkle shoots in collection was used to produce infected plants in pots that were separated according to the diverse symptomatology i.e. phyllody and witches’ broom. Small RNA high-throughput sequencing (HTS) was then used to determine the small RNA pattern of these plants. Bioinformatics analysis revealed the presence of expression changes of different miRNA classes and the presence of phytoplasma derived small RNAs. These results could complement previous studies and serve as a starting point for small RNA omics in phytoplasma research
Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons). Two-condition experiment, phytoplasma-infected plant and phytoplasma-infected insect. Biological replicates: 6 phytoplasma-infected plants and 6 phytoplasma-infected insects, independently grown and harvested. One replicate per array.
Project description:Transcriptional profiling of phytoplasma grown in plant (Chrysanthemum coronarium) and grown in insect (Macrosteles striifrons). Two-condition experiment, phytoplasma-infected plant and phytoplasma-infected insect. Biological replicates: 4 phytoplasma-infected plants and 4 phytoplasma-infected insects, independently grown and harvested. One replicate per array.
