Project description:Because of their small size, nanoparticles that enter the human body can easily penetrate biological barriers and can be circulated throughout the entire body, ultimately reaching the vascular endothelium. In this study aimed to identify cell reponses by distinguishing endothelial cell and exposing them to silver nanoparticles. The study also assesseed several gene expression levels that increased significantly in the microarray assay. We verified through microarray that 5 nm silver nanoparticles affect the variation of gene expression in cells, and a noticeable increase in the expression of interleukin (IL)-8 and IL-11 genes in early time was also verified. This study found that the variation on oxidative stress related genes in early time, amd among them the variation of metallothionein(MT), heme oxygenase 1(HO1), and heat shock 70kDa protein(HSP70) expression, was noticeable. This study finds that intracellular genes specifically respond to exposure to silver nanoparticles and that the expression of IL-11 among cytokines is noticeable.
